Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMK59_RS04640 Genome accession   NZ_CP101302
Coordinates   977214..977654 (-) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain 2000-3039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972214..982654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK59_RS04615 (NMK59_04615) coaE 972704..973324 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  NMK59_RS04620 (NMK59_04620) - 973349..973393 (+) 45 WP_120795372.1 protein YacM -
  NMK59_RS04625 (NMK59_04625) guaC 973549..974592 (+) 1044 WP_001217338.1 GMP reductase -
  NMK59_RS04630 (NMK59_04630) hofC 974627..975829 (-) 1203 WP_000157234.1 protein transport protein HofC -
  NMK59_RS04635 (NMK59_04635) pilB 975819..977204 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  NMK59_RS04640 (NMK59_04640) pilA 977214..977654 (-) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  NMK59_RS04645 (NMK59_04645) nadC 977857..978750 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMK59_RS04650 (NMK59_04650) ampD 978838..979389 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMK59_RS04655 (NMK59_04655) ampE 979386..980240 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMK59_RS04660 (NMK59_04660) aroP 980283..981656 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=610180 NMK59_RS04640 WP_000360894.1 977214..977654(-) (pilA) [Escherichia coli strain 2000-3039]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=610180 NMK59_RS04640 WP_000360894.1 977214..977654(-) (pilA) [Escherichia coli strain 2000-3039]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377