Detailed information NTG0609840

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: NML18_RS22100
• Genome accession: NZ_CP101269
• Coordinates: 4554322..4556520 (+)
• Length: 732 a.a.
• NCBI ID: WP_254837342.1
• Uniprot ID: -
• Organism: Escherichia coli strain EC21Z-048
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4549322..4561520

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NML18_RS22090 (NML18_22095)	rhsC	4549575..4553747 (-)	4173	WP_254837341.1	rhs element protein RhsC	-
NML18_RS22095 (NML18_22100)	rpmE	4553907..4554119 (-)	213	WP_000710769.1	50S ribosomal protein L31	-
NML18_RS22100 (NML18_22105)	priA	4554322..4556520 (+)	2199	WP_254837342.1	primosomal protein N'	Machinery gene
NML18_RS22105 (NML18_22110)	cytR	4556676..4557701 (+)	1026	WP_104725685.1	DNA-binding transcriptional regulator CytR	Regulator
NML18_RS22110 (NML18_22115)	ftsN	4557793..4558752 (+)	960	WP_000068830.1	cell division protein FtsN	-
NML18_RS22115 (NML18_22120)	hslV	4558845..4559375 (+)	531	WP_000208242.1	ATP-dependent protease subunit HslV	-
NML18_RS22120 (NML18_22125)	hslU	4559385..4560716 (+)	1332	WP_001293343.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 732 a.a.
• Molecular weight: 81701.96 Da
• Isoelectric Point: 8.9000

>NTDB_id=609840 NML18_RS22100 WP_254837342.1 4554322..4556520(+) (priA) [Escherichia coli strain EC21Z-048]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTISRFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPDVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHYPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide

• Length: 2199 bp

>NTDB_id=609840 NML18_RS22100 WP_254837342.1 4554322..4556520(+) (priA) [Escherichia coli strain EC21Z-048]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTGGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCCCCCAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTACGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCA
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCTTGACACCGCAAACTATCTCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGGCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCATAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGCCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGATGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACACAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCTCAGCTTTACACCCAGGTCGCTGGTCGTGCCGGGCG
TGCGGGTAAACAAGGCGAAGTGGTGCTGCAAACGCACTATCCAGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGGATGATGCAACTGCCGCCATGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTACTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (632-729)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (445-471)

• Helicase conserved C-terminal domain (Helicase_C) (522-589)

• DEAD/DEAH box helicase (DEAD) (203-362)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-101)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	45.047	100	0.454