Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   Dongsha4_RS17295 Genome accession   NZ_CP084098
Coordinates   3972542..3974563 (-) Length   673 a.a.
NCBI ID   WP_330205468.1    Uniprot ID   -
Organism   Cyanobacterium sp. Dongsha4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3967542..3979563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Dongsha4_RS17270 (Dongsha4_17235) - 3968187..3968693 (+) 507 WP_330203527.1 cupin domain-containing protein -
  Dongsha4_RS17275 (Dongsha4_17240) - 3969098..3969874 (+) 777 WP_330203528.1 DUF1868 domain-containing protein -
  Dongsha4_RS17280 (Dongsha4_17245) - 3969882..3970508 (+) 627 WP_330203529.1 precorrin-8X methylmutase -
  Dongsha4_RS17285 (Dongsha4_17250) rsmD 3970512..3971060 (+) 549 WP_330203530.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  Dongsha4_RS17290 (Dongsha4_17255) - 3971100..3972245 (-) 1146 WP_330203531.1 SpoIIE family protein phosphatase -
  Dongsha4_RS17295 (Dongsha4_17260) pilB/pilB1 3972542..3974563 (-) 2022 WP_330205468.1 GspE/PulE family protein Machinery gene
  Dongsha4_RS17305 (Dongsha4_17270) - 3975047..3976450 (+) 1404 WP_330203532.1 CAP domain-containing protein -
  Dongsha4_RS17310 (Dongsha4_17275) - 3976465..3976893 (+) 429 WP_330203533.1 VOC family protein -
  Dongsha4_RS17315 (Dongsha4_17280) - 3976873..3977007 (-) 135 WP_330203534.1 hypothetical protein -
  Dongsha4_RS17320 (Dongsha4_17285) - 3977184..3977870 (-) 687 WP_330203535.1 hypothetical protein -
  Dongsha4_RS17325 (Dongsha4_17290) - 3978275..3978770 (+) 496 Protein_3412 hypothetical protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75179.13 Da        Isoelectric Point: 4.9555

>NTDB_id=609530 Dongsha4_RS17295 WP_330205468.1 3972542..3974563(-) (pilB/pilB1) [Cyanobacterium sp. Dongsha4]
MSSASKEKLKTALVSPDYFNPFGNKLIQAGYIDKEDLKRALVQVRNTKQPLVKVLEQITHKALSPELVRQYKQHQLFELK
ILYGVDCVDPESEEIAADQMTDLIESIMGVQVCRRHKLLPLRRLEGEPPVIVIAMVNPDDLQALDDVKKILRAKGLGLQR
IVITEEDFDKLINKFYEEDKQRQKREEELKERQKLDQLGDVSDIVDDLDQDLDEAEDENPDGDLTSNDANQAPIIKLVNK
ILIKALQEGASDIHVEPQETRMRVRLRKDGVLREYFTLPKHITTAVVARFKIMAELDIAERRLPQDGKIRRVFQGRNVDF
RVNCLPSRYGEKVCLRILDNSATQLGLDFLISDEETLAKVRDIASRPFGLILVTGPTGSGKSTTLYSVLAERNDPGVNIS
TAEDPIEYALEGITQVQVIREKGMDFASILRAFLRQDPDIILVGETRDAETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMISGALLGVLAQRLMRRVCSECKVAYTPSKEELARFGLSVSGEESVTFYKANTLSGEVLQQAQANGTVCQK
CGGGGYKGRVGVYEFMVMSEELQKLINRGATTDMIKEKAVEEGMVTILAYSLNLVRQGHTTLEEVERVTFTDSGLEAELK
AKRKSSLTCSGCGAELQQEWLDCPYCMTPRFSE

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=609530 Dongsha4_RS17295 WP_330205468.1 3972542..3974563(-) (pilB/pilB1) [Cyanobacterium sp. Dongsha4]
ATTTCTTCTGCTTCTAAAGAAAAACTCAAAACAGCCTTAGTAAGTCCAGATTATTTCAATCCCTTCGGAAATAAGCTAAT
TCAAGCAGGTTACATTGATAAGGAAGATTTAAAAAGGGCGTTGGTACAAGTGAGAAATACTAAACAACCTTTGGTTAAAG
TCCTAGAACAAATTACCCATAAGGCTTTGTCTCCTGAGTTAGTACGACAGTACAAACAACATCAACTTTTTGAGTTAAAA
ATCCTTTATGGGGTTGATTGTGTTGACCCAGAGTCGGAAGAAATAGCGGCGGATCAAATGACCGATTTAATCGAGTCTAT
TATGGGAGTTCAGGTTTGTCGTCGTCATAAACTTTTACCCTTGCGTCGCCTTGAAGGTGAACCGCCTGTAATTGTGATTG
CTATGGTTAATCCTGATGATTTACAGGCGTTAGATGATGTCAAAAAAATATTACGAGCAAAAGGTCTTGGCTTACAAAGA
ATTGTTATTACCGAAGAAGATTTTGATAAGTTAATTAATAAGTTTTATGAAGAAGATAAGCAAAGACAAAAAAGGGAAGA
GGAGTTAAAAGAAAGGCAAAAATTAGATCAGTTAGGGGATGTTAGCGATATTGTCGATGACTTGGATCAGGATTTAGATG
AAGCTGAAGACGAAAATCCAGATGGTGATTTGACTTCTAATGATGCTAATCAAGCTCCTATTATCAAGTTAGTTAATAAA
ATTCTCATCAAGGCTTTACAAGAGGGAGCATCAGATATTCATGTTGAACCCCAAGAAACCAGAATGAGGGTGCGTTTACG
CAAAGACGGGGTTTTAAGGGAATATTTTACTCTGCCTAAACATATTACAACTGCGGTGGTAGCTCGTTTCAAAATTATGG
CTGAGTTGGATATTGCGGAACGGCGTTTACCTCAAGATGGCAAGATTCGTCGGGTGTTTCAGGGAAGAAATGTGGATTTT
CGGGTCAACTGTTTGCCCAGTCGTTATGGTGAAAAGGTCTGTTTAAGGATTTTAGATAATTCGGCTACTCAATTAGGTTT
AGACTTTTTAATCTCTGATGAGGAAACATTGGCAAAAGTTAGAGATATTGCTAGTCGTCCTTTTGGTTTAATTCTGGTTA
CAGGTCCTACGGGTTCGGGTAAATCCACAACTCTCTATTCTGTTTTGGCGGAAAGAAATGATCCGGGGGTAAACATCAGC
ACTGCAGAAGACCCCATTGAATATGCTTTGGAAGGAATCACTCAGGTGCAGGTAATCCGAGAAAAAGGTATGGATTTTGC
TTCGATTCTTCGGGCTTTCCTCAGACAAGACCCTGATATTATTCTGGTGGGGGAAACACGGGACGCTGAAACGGCAAAAA
CTGCGATCGAAGCCGCTTTGACTGGACACTTGGTTTTAACCACTCTACATACTAATGATGCCGCAGGTGCGATCGCACGT
TTGGATGAAATGGGGGTTGAACCCTTTATGATTTCTGGTGCATTGTTAGGAGTTTTAGCACAAAGATTAATGCGAAGAGT
TTGTAGTGAGTGTAAAGTTGCCTATACACCTAGTAAAGAAGAATTAGCCCGTTTTGGTTTGTCTGTATCTGGGGAAGAAT
CTGTGACTTTTTATAAAGCCAATACCTTGTCAGGAGAAGTCCTACAACAAGCCCAAGCTAATGGCACGGTGTGCCAAAAA
TGTGGTGGTGGTGGCTATAAAGGGCGTGTGGGTGTTTATGAGTTTATGGTGATGAGTGAAGAGTTACAAAAACTAATTAA
CCGAGGGGCAACCACTGATATGATTAAAGAAAAGGCTGTTGAAGAAGGAATGGTAACGATTTTAGCCTATAGTTTAAATT
TAGTTCGTCAAGGTCATACTACCCTAGAAGAAGTTGAAAGGGTAACATTTACTGATTCTGGTTTGGAAGCGGAATTAAAA
GCTAAACGAAAATCATCTTTAACCTGTAGTGGCTGTGGAGCAGAATTACAACAGGAATGGTTAGATTGCCCTTATTGTAT
GACTCCTCGCTTCAGTGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-180)

Type II/IV secretion system protein (T2SSE)

(240-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.798

97.623

0.672
  pilF Thermus thermophilus HB27

41.374

93.016

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.968

93.314

0.373