Detailed information NTG0060889

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: CYCME_RS03160
• Genome accession: NC_021917
• Coordinates: 640427..641425 (-)
• Length: 332 a.a.
• NCBI ID: WP_016390575.1
• Uniprot ID: S5TDV1
• Organism: Cycloclasticus zancles 78-ME
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 635427..646425

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CYCME_RS03135 (CYCME_0641)	cyoE	636296..637186 (+)	891	WP_020932131.1	heme o synthase	-
CYCME_RS03140 (CYCME_0642)	-	637285..637809 (+)	525	WP_041230537.1	retropepsin-like aspartic protease family protein	-
CYCME_RS03145 (CYCME_0643)	rpoH	637832..638701 (-)	870	WP_015006614.1	RNA polymerase sigma factor RpoH	-
CYCME_RS03150 (CYCME_0644)	ftsX	638807..639766 (-)	960	WP_016390574.1	permease-like cell division protein FtsX	-
CYCME_RS03155 (CYCME_0645)	ftsE	639759..640421 (-)	663	WP_015006612.1	cell division ATP-binding protein FtsE	-
CYCME_RS03160 (CYCME_0646)	pilA	640427..641425 (-)	999	WP_016390575.1	signal recognition particle-docking protein FtsY	Machinery gene
CYCME_RS03165 (CYCME_0647)	-	641502..642854 (+)	1353	WP_016390576.1	M16 family metallopeptidase	-
CYCME_RS03170 (CYCME_0648)	-	642844..644163 (+)	1320	WP_020932132.1	M16 family metallopeptidase	-
CYCME_RS03175 (CYCME_0649)	rsmD	644163..644744 (+)	582	WP_020932133.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CYCME_RS03180 (CYCME_0650)	coaD	644737..645216 (+)	480	WP_015006607.1	pantetheine-phosphate adenylyltransferase	-
CYCME_RS03185 (CYCME_0651)	-	645221..645478 (+)	258	WP_015006606.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 36059.18 Da
• Isoelectric Point: 5.4794

>NTDB_id=60889 CYCME_RS03160 WP_016390575.1 640427..641425(-) (pilA) [Cycloclasticus zancles 78-ME]
MFGFGKKKDTDTTKQPPVEEPKEGFFGRLKNQLTKTRSGLTGGLGNLFLGKKQIDDDLFEELEMLLLTADVGIEATQHII
SNLTQRVARKQLADPQALTQALQEEMLSILEPNQQALTIPDSDGPFVILVVGVNGMGKTTTIGKLAKRLQAEGKSVMLAA
GDTFRAAAIEQLQEWGKRNDVEVVAQHTGADSASVIYDAFEKAKARNIDVLLADTAGRLHTQTNLMNELSKINRVIKKLD
DSAPHETMLVLDAGTGQNALAQAKNFDKATPLTSITLTKLDGTAKGGIIFAIANQFKLPIRFIGIGEGINDLRPFDAAEF
TTALFEKSETDH

Nucleotide

• Length: 999 bp

>NTDB_id=60889 CYCME_RS03160 WP_016390575.1 640427..641425(-) (pilA) [Cycloclasticus zancles 78-ME]
ATGTTTGGATTTGGCAAAAAGAAAGATACCGACACAACTAAGCAGCCGCCTGTCGAAGAACCTAAAGAAGGTTTTTTTGG
CCGCTTAAAAAACCAGTTAACAAAAACACGCAGCGGATTGACCGGTGGTTTAGGTAATTTATTTTTAGGGAAAAAACAAA
TCGACGACGATTTGTTTGAAGAATTAGAAATGTTACTGCTAACAGCTGATGTCGGCATTGAAGCCACTCAACACATCATT
TCAAATTTAACTCAGCGAGTTGCGCGCAAGCAGCTAGCCGACCCACAGGCTCTCACCCAAGCACTTCAAGAAGAGATGCT
ATCGATCCTTGAGCCAAACCAACAAGCTTTAACCATTCCTGACAGTGACGGCCCTTTCGTTATCTTAGTGGTCGGCGTTA
ATGGCATGGGTAAAACGACGACCATTGGTAAACTGGCTAAACGTTTACAAGCCGAGGGCAAAAGTGTCATGTTAGCGGCT
GGCGATACATTTAGGGCCGCTGCGATTGAACAATTACAAGAATGGGGGAAGCGTAACGACGTTGAGGTTGTTGCTCAACA
TACTGGGGCTGACTCTGCTTCGGTTATTTACGATGCCTTTGAGAAGGCAAAAGCGCGCAACATTGACGTCTTACTGGCAG
ACACTGCTGGCCGCCTACATACCCAAACAAACCTAATGAATGAGTTAAGCAAAATCAACCGTGTTATTAAAAAACTGGAT
GACAGCGCGCCACACGAAACCATGCTGGTTCTTGATGCAGGCACCGGACAAAATGCCCTCGCCCAAGCTAAGAATTTTGA
TAAAGCCACCCCACTAACTAGCATCACTCTTACGAAATTGGACGGCACCGCAAAAGGTGGTATTATTTTCGCCATTGCCA
ATCAATTCAAACTACCCATTCGTTTTATTGGTATTGGTGAAGGCATCAATGACCTACGTCCTTTTGATGCCGCCGAGTTC
ACCACGGCTCTATTTGAAAAATCTGAAACTGACCATTAG

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (125-325)

• SRP54-type protein, helical bundle domain (SRP54_N) (40-106)

3D structure

• AlphaFold DB: S5TDV1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.896	92.771	0.5