Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD33_RS19005 Genome accession   NZ_CP100981
Coordinates   3887848..3888288 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET220     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3882848..3893288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD33_RS18985 (NMD33_18870) aroP 3883846..3885219 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD33_RS18990 (NMD33_18875) ampE 3885262..3886116 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD33_RS18995 (NMD33_18880) ampD 3886113..3886664 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD33_RS19000 (NMD33_18885) nadC 3886752..3887645 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD33_RS19005 (NMD33_18890) pilA 3887848..3888288 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD33_RS19010 (NMD33_18895) pilB 3888298..3889683 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD33_RS19015 (NMD33_18900) hofC 3889673..3890875 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD33_RS19020 (NMD33_18905) guaC 3890910..3891953 (-) 1044 WP_001217338.1 GMP reductase -
  NMD33_RS19025 (NMD33_18910) - 3892109..3892153 (-) 45 WP_120795372.1 protein YacM -
  NMD33_RS19030 (NMD33_18915) coaE 3892178..3892798 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=608203 NMD33_RS19005 WP_000360904.1 3887848..3888288(+) (pilA) [Escherichia coli strain ET220]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=608203 NMD33_RS19005 WP_000360904.1 3887848..3888288(+) (pilA) [Escherichia coli strain ET220]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363