Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LAZ96_RS12035 Genome accession   NZ_CP083715
Coordinates   2322061..2322498 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain DMB05     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2317061..2327498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ96_RS12010 (LAZ96_12010) - 2318081..2319217 (+) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -
  LAZ96_RS12015 (LAZ96_12015) - 2319232..2319666 (+) 435 WP_014416871.1 RDD family protein -
  LAZ96_RS12020 (LAZ96_12020) - 2319783..2320061 (+) 279 WP_263612330.1 YckD family protein -
  LAZ96_RS12025 (LAZ96_12025) - 2320165..2321601 (+) 1437 WP_104843069.1 glycoside hydrolase family 1 protein -
  LAZ96_RS12030 (LAZ96_12030) nin/comJ 2321642..2322040 (-) 399 WP_017419379.1 competence protein ComJ Regulator
  LAZ96_RS12035 (LAZ96_12035) nucA/comI 2322061..2322498 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LAZ96_RS12040 (LAZ96_12040) hxlB 2322829..2323386 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  LAZ96_RS12045 (LAZ96_12045) hxlA 2323383..2324018 (-) 636 WP_123117601.1 3-hexulose-6-phosphate synthase -
  LAZ96_RS12050 (LAZ96_12050) - 2324250..2324612 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=607419 LAZ96_RS12035 WP_003156588.1 2322061..2322498(-) (nucA/comI) [Bacillus velezensis strain DMB05]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=607419 LAZ96_RS12035 WP_003156588.1 2322061..2322498(-) (nucA/comI) [Bacillus velezensis strain DMB05]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGTTACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641