Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K9N54_RS15355 Genome accession   NZ_CP083361
Coordinates   3190423..3191439 (-) Length   338 a.a.
NCBI ID   WP_025793368.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 20-082E4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3185423..3196439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N54_RS15330 (K9N54_15215) aroK 3186283..3186801 (-) 519 WP_005381319.1 shikimate kinase AroK -
  K9N54_RS15335 (K9N54_15220) pilQ 3186990..3188738 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  K9N54_RS15340 (K9N54_15225) pilP 3188770..3189285 (-) 516 WP_023585576.1 pilus assembly protein PilP Machinery gene
  K9N54_RS15345 (K9N54_15230) pilO 3189275..3189868 (-) 594 WP_025793367.1 type 4a pilus biogenesis protein PilO Machinery gene
  K9N54_RS15350 (K9N54_15235) pilN 3189861..3190439 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  K9N54_RS15355 (K9N54_15240) pilM 3190423..3191439 (-) 1017 WP_025793368.1 type IV pilus biogenesis protein PilM Machinery gene
  K9N54_RS15360 (K9N54_15245) - 3191582..3194152 (+) 2571 WP_005480476.1 penicillin-binding protein 1A -
  K9N54_RS15365 (K9N54_15250) oxyR 3194208..3195116 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  K9N54_RS15370 (K9N54_15255) - 3195287..3196015 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37780.32 Da        Isoelectric Point: 6.8323

>NTDB_id=605390 K9N54_RS15355 WP_025793368.1 3190423..3191439(-) (pilM) [Vibrio parahaemolyticus strain 20-082E4]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMMAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=605390 K9N54_RS15355 WP_025793368.1 3190423..3191439(-) (pilM) [Vibrio parahaemolyticus strain 20-082E4]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGATGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ATCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.071

99.704

0.479