Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K8O83_RS03265 Genome accession   NZ_CP082857
Coordinates   575141..575680 (-) Length   179 a.a.
NCBI ID   WP_006996133.1    Uniprot ID   -
Organism   Haemophilus aegyptius strain FDAARGOS_1478     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570141..580680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O83_RS03235 (K8O83_03180) fdx 570544..570885 (+) 342 WP_006996128.1 ISC system 2Fe-2S type ferredoxin -
  K8O83_RS03240 (K8O83_03185) iscX 570885..571079 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  K8O83_RS03245 (K8O83_03190) - 571130..571744 (-) 615 WP_006996129.1 YfgM family protein -
  K8O83_RS03250 (K8O83_03195) hisS 571762..573033 (-) 1272 WP_006996130.1 histidine--tRNA ligase -
  K8O83_RS03255 (K8O83_03200) ispG 573043..574149 (-) 1107 WP_006996131.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K8O83_RS03260 (K8O83_03205) - 574158..575069 (-) 912 WP_006996132.1 RodZ family helix-turn-helix domain-containing protein -
  K8O83_RS03265 (K8O83_03210) pilF/pilF2 575141..575680 (-) 540 WP_006996133.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K8O83_RS03270 (K8O83_03215) - 575781..576932 (-) 1152 WP_013527479.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K8O83_RS03275 (K8O83_03220) - 577179..578057 (+) 879 WP_005662660.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K8O83_RS10230 - 578049..578174 (-) 126 WP_257005528.1 hypothetical protein -
  K8O83_RS03280 (K8O83_03225) - 578422..579303 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  K8O83_RS03285 (K8O83_03230) - 579303..580223 (+) 921 WP_006996135.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20531.12 Da        Isoelectric Point: 8.2626

>NTDB_id=603117 K8O83_RS03265 WP_006996133.1 575141..575680(-) (pilF/pilF2) [Haemophilus aegyptius strain FDAARGOS_1478]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=603117 K8O83_RS03265 WP_006996133.1 575141..575680(-) (pilF/pilF2) [Haemophilus aegyptius strain FDAARGOS_1478]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGCGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCGGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453