Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   K8P04_RS02915 Genome accession   NZ_CP082856
Coordinates   594874..595413 (+) Length   179 a.a.
NCBI ID   WP_077643706.1    Uniprot ID   -
Organism   Haemophilus influenzae strain FDAARGOS_1479     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589874..600413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8P04_RS02895 (K8P04_02870) - 590553..591473 (-) 921 WP_223364817.1 ATP-binding cassette domain-containing protein -
  K8P04_RS02900 (K8P04_02875) - 591473..592354 (-) 882 WP_015701596.1 metal ABC transporter substrate-binding protein -
  K8P04_RS02905 (K8P04_02880) - 592497..593375 (-) 879 WP_223364818.1 D-alanyl-D-alanine carboxypeptidase family protein -
  K8P04_RS02910 (K8P04_02885) - 593622..594773 (+) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  K8P04_RS02915 (K8P04_02890) pilF/pilF2 594874..595413 (+) 540 WP_077643706.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K8P04_RS02920 (K8P04_02895) - 595485..596396 (+) 912 WP_077643705.1 RodZ family helix-turn-helix domain-containing protein -
  K8P04_RS02925 (K8P04_02900) ispG 596405..597511 (+) 1107 WP_077643704.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K8P04_RS02930 (K8P04_02905) hisS 597521..598792 (+) 1272 WP_013525573.1 histidine--tRNA ligase -
  K8P04_RS02935 (K8P04_02910) - 598810..599424 (+) 615 WP_077643703.1 YfgM family protein -
  K8P04_RS02940 (K8P04_02915) iscX 599475..599669 (-) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  K8P04_RS02945 (K8P04_02920) fdx 599669..600010 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20560.15 Da        Isoelectric Point: 7.8634

>NTDB_id=603079 K8P04_RS02915 WP_077643706.1 594874..595413(+) (pilF/pilF2) [Haemophilus influenzae strain FDAARGOS_1479]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKDYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=603079 K8P04_RS02915 WP_077643706.1 594874..595413(+) (pilF/pilF2) [Haemophilus influenzae strain FDAARGOS_1479]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAAAGCACTTCAACACGATAAAGATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447