Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   K8B68_RS07250 Genome accession   NZ_CP082813
Coordinates   1536682..1537656 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain SCAID OTT1-2021 (597/2)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1531682..1542656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B68_RS07210 (K8B68_07210) gcvT 1532183..1533274 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  K8B68_RS07215 (K8B68_07215) - 1533433..1533957 (-) 525 WP_001015120.1 shikimate kinase -
  K8B68_RS07220 (K8B68_07220) - 1533947..1534093 (-) 147 WP_001789879.1 hypothetical protein -
  K8B68_RS07225 (K8B68_07225) comGF 1534190..1534687 (-) 498 WP_029694423.1 competence type IV pilus minor pilin ComGF Machinery gene
  K8B68_RS07230 (K8B68_07230) comGE 1534623..1534904 (-) 282 WP_000844412.1 hypothetical protein Machinery gene
  K8B68_RS07235 (K8B68_07235) comGD 1534891..1535337 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  K8B68_RS07240 (K8B68_07240) comGC 1535315..1535626 (-) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  K8B68_RS07245 (K8B68_07245) comGB 1535640..1536710 (-) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  K8B68_RS07250 (K8B68_07250) comGA 1536682..1537656 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  K8B68_RS07255 (K8B68_07255) - 1537708..1538331 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  K8B68_RS07260 (K8B68_07260) - 1538328..1538657 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  K8B68_RS07265 (K8B68_07265) - 1538657..1539643 (-) 987 WP_000161307.1 ROK family glucokinase -
  K8B68_RS07270 (K8B68_07270) - 1539640..1539843 (-) 204 WP_000087561.1 YqgQ family protein -
  K8B68_RS07275 (K8B68_07275) - 1539824..1541287 (-) 1464 WP_001017870.1 rhomboid family protein -
  K8B68_RS07280 (K8B68_07280) - 1541299..1541838 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  K8B68_RS07285 (K8B68_07285) rpmG 1542047..1542196 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=602452 K8B68_RS07250 WP_000697220.1 1536682..1537656(-) (comGA) [Staphylococcus aureus strain SCAID OTT1-2021 (597/2)]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=602452 K8B68_RS07250 WP_000697220.1 1536682..1537656(-) (comGA) [Staphylococcus aureus strain SCAID OTT1-2021 (597/2)]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1