Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K6959_RS17285 Genome accession   NZ_CP082780
Coordinates   3564832..3566214 (+) Length   460 a.a.
NCBI ID   WP_163241581.1    Uniprot ID   A0A6B3VYE7
Organism   Bacillus aquiflavi strain 3H-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3559832..3571214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6959_RS17270 (K6959_17270) - 3560049..3560597 (+) 549 WP_223087149.1 UvrB/UvrC motif-containing protein -
  K6959_RS17275 (K6959_17275) - 3560601..3561671 (+) 1071 WP_223088425.1 protein arginine kinase -
  K6959_RS17280 (K6959_17280) clpC 3561688..3564123 (+) 2436 WP_223087151.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  K6959_RS17285 (K6959_17285) radA 3564832..3566214 (+) 1383 WP_163241581.1 DNA repair protein RadA Machinery gene
  K6959_RS17290 (K6959_17290) - 3566678..3567760 (+) 1083 WP_223087152.1 PIN/TRAM domain-containing protein -
  K6959_RS17295 (K6959_17295) ispD 3567780..3568502 (+) 723 WP_163241585.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  K6959_RS17300 (K6959_17300) ispF 3568477..3568956 (+) 480 WP_163241723.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  K6959_RS17305 (K6959_17305) gltX 3569050..3570507 (+) 1458 Protein_3468 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 50256.86 Da        Isoelectric Point: 7.3635

>NTDB_id=602169 K6959_RS17285 WP_163241581.1 3564832..3566214(+) (radA) [Bacillus aquiflavi strain 3H-10]
MSKRKTKYICQECGYESPKWMGKCPGCNQWNKMVEEIEHTGRGRRGAFAHSYDDSSVLSKATPITSIETVSEPRIQTELI
ELNRVLGGGVVKGSLILIGGDPGIGKSTLLLQVSAQLAKKGQSVLYISGEESLKQTKLRADRLKVSSDTLLVYSETNLEE
INRTIEKMNPNCVIVDSIQTVFHPDVTSAPGSVSQVRECTAELMRIAKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVL
YFEGERHHTYRILRTVKNRFGSTNEIGIFEMKELGLEEVANPSEIFLEERSEGASGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVCIASSFRDKPTKPSDCIIGEVG
LTGEVRRVSRIEQRVNEAAKLGFERVILPMNNLGGWRGPKGIELIGVSSISEALQVTLEG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=602169 K6959_RS17285 WP_163241581.1 3564832..3566214(+) (radA) [Bacillus aquiflavi strain 3H-10]
ATGTCAAAGCGGAAAACGAAATACATTTGTCAAGAATGTGGTTATGAATCTCCAAAGTGGATGGGAAAATGTCCAGGATG
CAACCAGTGGAATAAAATGGTTGAAGAAATTGAGCATACAGGAAGAGGTAGACGGGGGGCTTTTGCGCATTCTTATGATG
ATAGTAGTGTATTATCAAAAGCAACACCCATTACATCAATAGAAACGGTATCTGAGCCGCGAATTCAGACAGAACTCATT
GAATTAAACCGAGTTCTTGGCGGCGGTGTTGTAAAAGGATCACTTATTTTAATCGGCGGTGACCCAGGAATTGGTAAATC
GACGTTACTTTTACAAGTTTCTGCTCAATTAGCTAAAAAAGGTCAGTCTGTCTTATATATTTCTGGCGAGGAATCGTTAA
AGCAAACGAAACTTCGAGCAGATCGGCTTAAGGTTTCTTCAGATACGCTCCTTGTTTATTCAGAGACGAATCTTGAGGAG
ATCAATAGAACAATTGAAAAAATGAATCCGAATTGTGTAATTGTTGATTCAATACAAACAGTTTTTCATCCAGATGTTAC
TTCTGCTCCAGGCAGTGTATCCCAAGTTCGTGAATGTACTGCAGAGTTGATGAGAATAGCTAAAACGAAAGGAATTGCGA
TCTTTATTGTAGGGCATGTAACGAAGGAAGGTTCGATTGCAGGTCCCCGGCTTTTAGAGCATATGGTAGATACCGTGCTT
TATTTTGAAGGGGAACGACATCATACATATCGCATTTTACGGACAGTGAAAAATCGTTTTGGTTCAACAAATGAAATAGG
GATATTTGAAATGAAAGAGCTTGGGCTTGAAGAGGTAGCGAATCCTTCAGAGATTTTTCTTGAAGAAAGATCAGAAGGCG
CATCTGGTTCGACTGTTGTTGCATCAATGGAAGGCACTCGCCCTGTTCTAGTAGAGATTCAGGCATTAATCTCTCCAACA
AGCTTCGGAAATCCAAGGCGAATGGCTACAGGTATTGATCATAATCGCGTTTCTCTTCTAATGGCAGTATTGGAAAAACG
TGTTGGTCTTTTATTACAAAATCAAGATGCGTATTTAAAAGTTGCGGGTGGAGTAAAATTAGATGAGCCTGCAATTGATT
TAGCAATTGCGGTCTGTATTGCTTCTAGTTTTCGAGATAAACCGACAAAACCTTCTGATTGTATTATAGGAGAAGTTGGT
TTAACAGGTGAAGTAAGAAGGGTATCAAGAATAGAACAAAGGGTTAACGAAGCGGCTAAATTAGGATTTGAAAGAGTCAT
TTTACCAATGAATAATCTTGGCGGCTGGAGAGGACCTAAAGGTATTGAGTTAATAGGTGTGTCTTCAATTAGTGAGGCTC
TTCAAGTAACTCTAGAGGGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(367-432)

AAA domain (AAA_25)

(67-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B3VYE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.007

100

0.772
  radA Streptococcus mitis NCTC 12261

62.198

98.913

0.615
  radA Streptococcus pneumoniae Rx1

62.198

98.913

0.615
  radA Streptococcus pneumoniae D39

62.198

98.913

0.615
  radA Streptococcus pneumoniae R6

62.198

98.913

0.615
  radA Streptococcus pneumoniae TIGR4

62.198

98.913

0.615
  radA Streptococcus mitis SK321

61.978

98.913

0.613