Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DVS74_RS06370 Genome accession   NZ_CP099516
Coordinates   1398343..1400043 (-) Length   566 a.a.
NCBI ID   WP_128283815.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain ESVL     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1393343..1405043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVS74_RS06340 (DVS74_006315) - 1393928..1394773 (-) 846 WP_004083682.1 general secretion pathway protein GspK -
  DVS74_RS06345 (DVS74_006320) - 1394773..1395399 (-) 627 WP_004083683.1 type II secretion system protein J -
  DVS74_RS06350 (DVS74_006325) xpsI 1395396..1395794 (-) 399 WP_004083684.1 type II secretion system protein XpsI -
  DVS74_RS06355 (DVS74_006330) xpsH 1395791..1396297 (-) 507 WP_004083685.1 type II secretion system protein XpsH -
  DVS74_RS06360 (DVS74_006335) gspG 1396303..1396737 (-) 435 WP_004083686.1 type II secretion system major pseudopilin GspG -
  DVS74_RS06365 (DVS74_006340) xpsF 1397090..1398307 (-) 1218 WP_128283812.1 type II secretion system protein XpsF -
  DVS74_RS06370 (DVS74_006345) pilF 1398343..1400043 (-) 1701 WP_128283815.1 type II secretion system ATPase GspE Machinery gene
  DVS74_RS06375 (DVS74_006350) - 1400336..1402783 (-) 2448 WP_004083689.1 YadA family autotransporter adhesin -
  DVS74_RS06380 (DVS74_006355) - 1403448..1403807 (-) 360 WP_004084115.1 AEC family transporter -
  DVS74_RS06385 (DVS74_006360) - 1403841..1404359 (-) 519 WP_004084214.1 hypothetical protein -
  DVS74_RS06390 (DVS74_006365) - 1404356..1404808 (-) 453 WP_004083690.1 TauD/TfdA family dioxygenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62695.50 Da        Isoelectric Point: 5.0845

>NTDB_id=602154 DVS74_RS06370 WP_128283815.1 1398343..1400043(-) (pilF) [Xylella fastidiosa subsp. multiplex strain ESVL]
MNVSMEASIVESLLQRRCLKESDLYRARQLQAESGVGLLPLLGRLGLVSERDHAEVCAEVMGLLLIQVRELPDTPPEFSA
EVHGLSIRFLKQFHVCPLAEHDGVLQLCVADPFDVYAIEALRLATGAVLQLCIGLRSEIDDLVERWYGQGRSVMGMIVEA
TDGDAAPADDIEALRDLASEAPVIRLVNLVIQRAVELRASDIHIEPFENRLKVRYRVDGLLIDAESPPSKLAAAVVSRVK
IMAKLNIAERRLPQDGRIMLRVQGKELDLRVSVVPTSHGESVVMRLLDRETVVFDFQKLGFTDELLNRFSRVLEMPHGIL
LVTGPTGSGKTTTLYTVLNRLNTTGVKIITVEDPVEYQIEGINQIQAKPQIGLDFASALRSIVRQDPDIIMIGEMRDLET
ARIAIQSALTGHLVLSTLHTNSAAGGLTRLLDMGVEDYLLTSTINGVLAQRLVRRLEPTHAERYLASPEQIARFDLRRLQ
PEGDIYLYRPRASALAPTGYLGRTAIVEFLIMDDVLRRAVISRTGIGELEQIARQAGMRTMYEDGLIKALAGETTIEEVL
RVTEEG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=602154 DVS74_RS06370 WP_128283815.1 1398343..1400043(-) (pilF) [Xylella fastidiosa subsp. multiplex strain ESVL]
ATGAATGTAAGTATGGAAGCATCCATCGTTGAGTCGTTGTTGCAGCGTCGTTGCTTGAAAGAAAGTGATCTGTATCGCGC
GCGTCAGTTGCAGGCCGAATCCGGTGTTGGGTTGTTACCTTTGTTGGGCCGTCTTGGTTTGGTTTCTGAGCGCGATCACG
CTGAAGTATGTGCGGAGGTGATGGGGCTGTTGTTGATACAGGTGCGTGAATTGCCGGATACGCCGCCAGAATTCTCGGCG
GAGGTGCATGGGTTGTCCATTCGCTTTCTCAAACAGTTCCATGTATGCCCGCTTGCTGAGCATGATGGTGTGTTGCAGTT
GTGTGTTGCAGATCCATTTGATGTGTACGCCATTGAGGCGTTGCGCCTTGCCACAGGTGCAGTATTGCAATTGTGTATCG
GTTTGCGTTCCGAAATTGATGACTTGGTCGAGCGCTGGTATGGCCAAGGCCGCAGCGTCATGGGCATGATTGTGGAGGCG
ACCGATGGTGATGCGGCGCCCGCAGATGACATTGAGGCTTTGCGTGATCTGGCGTCTGAAGCGCCTGTGATCCGGCTGGT
GAACTTGGTGATTCAGCGTGCGGTTGAATTACGCGCCTCTGATATCCATATCGAACCATTCGAGAATCGGTTGAAGGTAC
GTTATCGCGTCGATGGCTTGTTGATTGATGCGGAGAGTCCGCCATCTAAATTGGCTGCTGCTGTGGTTTCACGTGTCAAG
ATTATGGCCAAACTTAATATTGCTGAACGCCGCTTGCCCCAGGATGGGCGAATCATGTTGCGTGTTCAGGGTAAAGAGCT
GGATCTGCGGGTGAGTGTTGTGCCGACTTCACATGGTGAAAGCGTCGTGATGCGCCTGCTTGACCGGGAGACGGTGGTAT
TCGATTTTCAGAAGCTTGGTTTTACCGATGAGTTATTAAACAGGTTCTCCCGTGTGTTGGAGATGCCTCATGGCATTTTA
CTGGTCACGGGTCCGACGGGATCTGGAAAGACCACCACGCTGTATACGGTGTTAAATCGTCTCAATACTACCGGTGTCAA
GATTATTACGGTGGAAGATCCGGTTGAGTATCAGATTGAAGGTATCAATCAGATCCAGGCTAAGCCGCAGATCGGGCTGG
ATTTTGCCAGCGCACTACGCAGCATTGTGCGTCAGGATCCGGACATCATCATGATTGGTGAAATGCGGGATCTGGAAACT
GCGCGCATTGCTATCCAGTCCGCGCTGACGGGGCATTTGGTGCTGTCTACTTTGCATACCAATAGTGCTGCCGGTGGGCT
GACCCGATTACTGGATATGGGTGTGGAGGATTATTTGTTGACCTCCACAATCAATGGTGTTTTGGCACAGCGTTTGGTGC
GTCGCTTGGAGCCTACGCATGCTGAGCGTTATCTTGCTTCGCCAGAGCAGATTGCGCGTTTTGATTTGCGCCGTTTACAG
CCAGAGGGTGATATTTATTTGTACCGTCCGCGCGCTTCAGCGCTTGCGCCCACTGGGTATCTGGGCCGTACGGCGATCGT
CGAGTTTCTGATTATGGATGATGTGCTGCGTCGCGCGGTGATCAGCCGTACCGGCATAGGGGAGTTGGAGCAGATCGCAC
GTCAAGCAGGGATGCGTACTATGTACGAGGACGGTCTCATCAAGGCATTGGCTGGTGAGACTACAATTGAAGAGGTCTTG
CGCGTGACGGAGGAAGGCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

40.761

97.527

0.398
  pilB Vibrio campbellii strain DS40M4

40.516

95.936

0.389
  pilB Vibrio cholerae strain A1552

38.38

100

0.385
  pilB Vibrio parahaemolyticus RIMD 2210633

40.074

95.23

0.382
  pilB Legionella pneumophila strain ERS1305867

36.731

100

0.369