Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   K7B13_RS11915 Genome accession   NZ_CP082278
Coordinates   2362754..2363824 (-) Length   356 a.a.
NCBI ID   WP_115997636.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain 35M     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357754..2368824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7B13_RS11865 (K7B13_11865) sipW 2357813..2358397 (-) 585 WP_212098460.1 signal peptidase I SipW -
  K7B13_RS11870 (K7B13_11870) tapA 2358369..2359040 (-) 672 WP_212098462.1 amyloid fiber anchoring/assembly protein TapA -
  K7B13_RS11875 (K7B13_11875) - 2359298..2359627 (+) 330 WP_045510605.1 DUF3889 domain-containing protein -
  K7B13_RS11880 (K7B13_11880) - 2359668..2359847 (-) 180 WP_016938971.1 YqzE family protein -
  K7B13_RS11885 (K7B13_11885) comGG 2359904..2360281 (-) 378 WP_212098464.1 competence type IV pilus minor pilin ComGG Machinery gene
  K7B13_RS11890 (K7B13_11890) comGF 2360283..2360678 (-) 396 WP_212101733.1 competence type IV pilus minor pilin ComGF -
  K7B13_RS11895 (K7B13_11895) - 2360692..2360958 (-) 267 WP_258415241.1 type II secretion system protein -
  K7B13_RS11900 (K7B13_11900) comGD 2360990..2361427 (-) 438 WP_045510590.1 competence type IV pilus minor pilin ComGD Machinery gene
  K7B13_RS11905 (K7B13_11905) comGC 2361417..2361725 (-) 309 WP_115997634.1 competence type IV pilus major pilin ComGC Machinery gene
  K7B13_RS11910 (K7B13_11910) comGB 2361730..2362767 (-) 1038 WP_212098466.1 competence type IV pilus assembly protein ComGB Machinery gene
  K7B13_RS11915 (K7B13_11915) comGA 2362754..2363824 (-) 1071 WP_115997636.1 competence type IV pilus ATPase ComGA Machinery gene
  K7B13_RS11920 (K7B13_11920) - 2364017..2364967 (-) 951 WP_212098468.1 magnesium transporter CorA family protein -
  K7B13_RS11925 (K7B13_11925) - 2365114..2366415 (+) 1302 WP_212098471.1 hemolysin family protein -
  K7B13_RS11930 (K7B13_11930) - 2366453..2367283 (-) 831 WP_212098474.1 STAS domain-containing protein -
  K7B13_RS11940 (K7B13_11940) - 2367485..2367865 (-) 381 WP_115997640.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  K7B13_RS11945 (K7B13_11945) - 2368101..2368346 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40486.69 Da        Isoelectric Point: 8.8524

>NTDB_id=600869 K7B13_RS11915 WP_115997636.1 2362754..2363824(-) (comGA) [Bacillus amyloliquefaciens strain 35M]
MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSSGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCETDNCSVYCRLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=600869 K7B13_RS11915 WP_115997636.1 2362754..2363824(-) (comGA) [Bacillus amyloliquefaciens strain 35M]
ATGGAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC
GAGGGAAAGGGATGCTTTCATCCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAATTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT
CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
ATTGCCTAAGAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACTACCCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCTCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGTGCGGCCATGACGGGCCACCTCGTACTTTCAAGCCTGCAT
ACAAGAGATGCCAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTAATTGC
CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCGAAACGGACAACTGTTCCGTGTACTGCCGTCTGTACA
GGCAGACACGCCGTGCGGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAGGAAGCAAAAGGAGAG
CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGTATCGCGCTCGGTTATGTCACGACTGATAATTA
TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.584

100

0.826
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376
  pilF Thermus thermophilus HB27

37.892

98.596

0.374