Detailed information NTG0600570

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K4A81_RS13455
• Genome accession: NZ_CP082262
• Coordinates: 2715718..2716332 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain BIM B-454D
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2710718..2721332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K4A81_RS13425 (K4A81_13425)	rpsT	2711018..2711284 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
K4A81_RS13430 (K4A81_13430)	holA	2711301..2712342 (-)	1042	Protein_2625	DNA polymerase III subunit delta	-
K4A81_RS13435 (K4A81_13435)	-	2712382..2712536 (-)	155	Protein_2626	hypothetical protein	-
K4A81_RS13440 (K4A81_13440)	-	2712577..2712711 (+)	135	WP_003152870.1	YqzM family protein	-
K4A81_RS13445 (K4A81_13445)	comEC	2712730..2715081 (-)	2352	WP_103526739.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K4A81_RS13450 (K4A81_13450)	-	2715082..2715651 (-)	570	WP_003152868.1	ComE operon protein 2	-
K4A81_RS13455 (K4A81_13455)	comEA	2715718..2716332 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
K4A81_RS13460 (K4A81_13460)	comER	2716391..2717212 (+)	822	WP_029973401.1	late competence protein ComER	-
K4A81_RS13465 (K4A81_13465)	-	2717281..2718018 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
K4A81_RS13470 (K4A81_13470)	rsfS	2718015..2718371 (-)	357	WP_007408260.1	ribosome silencing factor	-
K4A81_RS13475 (K4A81_13475)	yqeK	2718389..2718949 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K4A81_RS13480 (K4A81_13480)	-	2718939..2719508 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
K4A81_RS13485 (K4A81_13485)	yhbY	2719519..2719809 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
K4A81_RS13490 (K4A81_13490)	aroE	2719803..2720645 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=600570 K4A81_RS13455 WP_014418419.1 2715718..2716332(-) (comEA) [Bacillus velezensis strain BIM B-454D]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=600570 K4A81_RS13455 WP_014418419.1 2715718..2716332(-) (comEA) [Bacillus velezensis strain BIM B-454D]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363