Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   K6751_RS05325 Genome accession   NZ_CP082244
Coordinates   1134425..1136020 (+) Length   531 a.a.
NCBI ID   WP_233473422.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain CSMC7.1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1129425..1141020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6751_RS05305 (K6751_05305) pgeF 1131213..1131938 (-) 726 WP_222901066.1 peptidoglycan editing factor PgeF -
  K6751_RS05310 (K6751_05310) rluD 1131938..1132909 (-) 972 WP_044410847.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  K6751_RS05315 (K6751_05315) - 1133057..1134055 (+) 999 WP_044410365.1 outer membrane protein assembly factor BamD -
  K6751_RS05320 (K6751_05320) - 1134202..1134438 (+) 237 WP_044410363.1 PP0621 family protein -
  K6751_RS05325 (K6751_05325) pilS 1134425..1136020 (+) 1596 WP_233473422.1 sensor histidine kinase Regulator
  K6751_RS05330 (K6751_05330) pilR 1136023..1137363 (+) 1341 WP_044410357.1 sigma-54-dependent transcriptional regulator Regulator
  K6751_RS05335 (K6751_05335) thiO 1137376..1138461 (-) 1086 WP_222901067.1 glycine oxidase ThiO -
  K6751_RS05340 (K6751_05340) - 1138490..1139017 (+) 528 WP_222901068.1 GspH/FimT family protein -
  K6751_RS05345 (K6751_05345) - 1139129..1139644 (+) 516 WP_222901069.1 GspH/FimT family pseudopilin -
  K6751_RS05350 (K6751_05350) pilV 1139631..1140206 (+) 576 WP_142012026.1 type IV pilus modification protein PilV -
  K6751_RS05355 (K6751_05355) - 1140203..1141006 (+) 804 WP_222901070.1 PilW family protein -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 59216.45 Da        Isoelectric Point: 8.1062

>NTDB_id=600497 K6751_RS05325 WP_233473422.1 1134425..1136020(+) (pilS) [Metapseudomonas otitidis strain CSMC7.1]
MASTESPFLASNQIRRIFRLYNLYRLLIGLALVGLISSELDSELLDLARVQLFRNASWFYLLLNILIALMVRRPQHMLQV
FSLALVDVILLSCIFYAAGGSPSGIGNLIIVAVAIANILLRGRIGLLIAAVAAIGMIYLTFYLSLSRPEAANQYVQAGAL
GALCFAGALFVQGISRRLQLSETLAEQRAADVANLEALNALILQRMRTGILVLDSRHRVLLANQAAQAMLGHDGLAGRPL
GPHCPELLKRLEQWWDNPTLRPQSLQPVPDGTVLQPSFVQLQRGEQLDTLVFLEDISQIAQQAQQLKLASLGRLTAGIAH
EIRNPLGAISHAAQLLQESEELEGPDRRLAQIIQDHSRRMNLVIENVLQLSRRRQSEPQLLDLKYWLHRFASEFRSASPV
GQTLHLETNPGSIQTRMDPHQLTQVLTNLVQNGLRYSAQKHRQGQVWLKLFRDAESDLPILEVMDDGPGVAEEQLHHLFE
PFHTTENKGTGLGLYISRELCESNQARLDYRPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=600497 K6751_RS05325 WP_233473422.1 1134425..1136020(+) (pilS) [Metapseudomonas otitidis strain CSMC7.1]
GTGGCGAGCACTGAGTCGCCCTTCCTGGCGAGCAATCAGATCCGACGGATCTTCCGCCTTTACAACCTGTACCGCCTGCT
CATCGGCCTGGCACTGGTCGGGCTGATTTCCAGTGAGCTGGACAGCGAGTTGCTGGATCTGGCTCGCGTGCAGCTATTCC
GCAATGCCAGCTGGTTCTACCTGCTGTTGAACATCCTCATTGCGCTGATGGTGCGCCGCCCCCAGCACATGCTGCAGGTG
TTCAGCCTGGCGCTGGTGGACGTTATCCTGCTGTCCTGCATCTTCTATGCAGCGGGCGGCTCGCCCAGCGGCATCGGCAA
CCTGATCATCGTTGCGGTGGCCATTGCCAACATCCTGCTGCGTGGGCGTATCGGGCTGCTGATCGCCGCCGTCGCCGCCA
TCGGGATGATCTACCTGACTTTCTACCTGAGCTTGTCCCGCCCGGAAGCGGCCAACCAGTACGTACAGGCCGGTGCGCTC
GGTGCCCTGTGCTTTGCAGGCGCCCTGTTCGTGCAGGGCATCAGCCGGCGCCTGCAGTTGAGCGAGACCCTGGCCGAGCA
GCGTGCTGCCGACGTGGCCAATCTGGAAGCGCTCAACGCGCTCATCCTGCAGCGCATGCGCACCGGCATCCTGGTGCTGG
ACAGCCGTCACCGGGTGTTGCTGGCCAACCAGGCTGCCCAGGCGATGCTGGGGCACGACGGGCTGGCGGGGCGCCCCCTC
GGGCCGCACTGCCCGGAGCTGCTCAAGCGACTGGAGCAGTGGTGGGACAACCCGACCCTGCGCCCCCAGAGCCTGCAGCC
GGTACCCGATGGCACGGTGCTGCAGCCCAGCTTCGTCCAGCTGCAGCGTGGTGAGCAGCTGGATACGCTGGTCTTCCTCG
AAGATATCTCGCAGATCGCCCAGCAGGCGCAGCAGCTGAAGCTGGCTTCCCTGGGGCGGCTGACCGCCGGTATCGCCCAT
GAGATCCGCAATCCCCTGGGCGCCATCAGTCATGCTGCCCAGCTGTTGCAGGAGTCCGAAGAGCTGGAGGGGCCCGACCG
CCGCCTGGCGCAGATCATCCAGGACCATTCCCGGCGGATGAACCTCGTCATCGAGAACGTCCTGCAACTGTCACGGCGCC
GCCAGTCGGAACCCCAACTGCTGGATCTCAAGTACTGGCTGCACCGATTCGCCAGCGAGTTCCGCAGCGCTTCACCGGTG
GGGCAGACCCTTCACCTGGAGACGAACCCGGGCAGTATCCAGACCCGCATGGACCCCCACCAGCTCACACAGGTGCTGAC
CAACCTGGTGCAGAACGGCCTGCGCTACAGCGCGCAGAAGCACCGCCAGGGGCAGGTCTGGCTGAAACTCTTCCGTGACG
CCGAGAGCGACCTGCCCATACTGGAAGTGATGGACGACGGCCCCGGCGTCGCCGAGGAGCAACTCCATCACCTGTTCGAG
CCCTTCCACACCACGGAGAACAAGGGCACCGGGCTTGGCCTGTACATCTCCCGCGAGCTGTGCGAGAGCAACCAGGCCCG
CCTTGACTATCGACCCCGCGAAGGCGGCGGCAGCTGTTTCCGCATCACCTTCGCCCATCCGCGAAAATTGAGTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(418-524)

PAS domain (PAS_8)

(203-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

67.686

98.493

0.667