Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   NBY12_RS01060 Genome accession   NZ_CP098223
Coordinates   219581..221779 (-) Length   732 a.a.
NCBI ID   WP_032304231.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0005     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 214581..226779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY12_RS01040 (NBY12_01040) hslU 215397..216728 (-) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -
  NBY12_RS01045 (NBY12_01045) hslV 216738..217268 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  NBY12_RS01050 (NBY12_01050) ftsN 217361..218308 (-) 948 WP_032304230.1 cell division protein FtsN -
  NBY12_RS01055 (NBY12_01055) cytR 218400..219425 (-) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  NBY12_RS01060 (NBY12_01060) priA 219581..221779 (-) 2199 WP_032304231.1 primosomal protein N' Machinery gene
  NBY12_RS01065 (NBY12_01065) rpmE 221982..222194 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  NBY12_RS01070 (NBY12_01070) yiiX 222255..222863 (-) 609 WP_032304233.1 YiiX family permuted papain-like enzyme -
  NBY12_RS01075 (NBY12_01075) metJ 222923..223240 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  NBY12_RS01080 (NBY12_01080) metB 223517..224677 (+) 1161 WP_001353720.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81618.94 Da        Isoelectric Point: 9.0294

>NTDB_id=598478 NBY12_RS01060 WP_032304231.1 219581..221779(-) (priA) [Escherichia coli strain Z0117EC0005]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPMFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQVYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=598478 NBY12_RS01060 WP_032304231.1 219581..221779(-) (priA) [Escherichia coli strain Z0117EC0005]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCCGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCACCCAAGCAACAACAGGCGCTGGCAGCGTTACGGCAAGGCAAAATCTGGCGTGATCAGGTTGCCACGCTCGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
CACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGTGTTACCGGTTCCGGTAAAACGGAAGTCTATTTAAGCGTGCTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATTGCCCGTTTTCGTGA
GCGGTTTAATGCGCCAGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGTGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAACAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACATTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTACGCCTGACCCGTCGGGCAGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCTGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGC
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCAATGTTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCATTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGGTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGTGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAACGACGCATGATGCAACTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCACGCGCCGCTGTTCCTGCAACAGTTGCGTAATCTGATCCTCGCCAGCCCGCTTGCAGA
CGACAAACTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCCCCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.505

100

0.448