Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   J450_RS09705 Genome accession   NC_021738
Coordinates   1971163..1971717 (+) Length   184 a.a.
NCBI ID   WP_006251773.1    Uniprot ID   -
Organism   Mannheimia haemolytica D171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1966163..1976717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J450_RS09675 (J450_09510) - 1966383..1967192 (+) 810 WP_006250123.1 metal ABC transporter permease -
  J450_RS12645 (J450_12450) - 1967249..1967991 (-) 743 Protein_1877 metal ABC transporter ATP-binding protein -
  J450_RS09685 (J450_09515) - 1968004..1968900 (-) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  J450_RS09690 (J450_09520) - 1969160..1970320 (+) 1161 WP_020831129.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  J450_RS09695 (J450_09525) - 1970361..1970708 (-) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  J450_RS09700 (J450_09530) - 1970692..1970943 (-) 252 WP_006251772.1 type II toxin-antitoxin system RelE family toxin -
  J450_RS09705 (J450_09535) pilF 1971163..1971717 (+) 555 WP_006251773.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  J450_RS09710 (J450_09540) - 1971717..1974041 (+) 2325 WP_006251774.1 Tex family protein -
  J450_RS09715 (J450_09545) - 1974112..1974675 (-) 564 WP_006249720.1 nitroreductase family protein -
  J450_RS09720 (J450_12210) sppA 1974783..1976638 (+) 1856 Protein_1885 signal peptide peptidase SppA -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20976.90 Da        Isoelectric Point: 7.0805

>NTDB_id=59807 J450_RS09705 WP_006251773.1 1971163..1971717(+) (pilF) [Mannheimia haemolytica D171]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=59807 J450_RS09705 WP_006251773.1 1971163..1971717(+) (pilF) [Mannheimia haemolytica D171]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446

Multiple sequence alignment