Detailed information NTG0596753

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K5A80_RS31870
• Genome accession: NZ_CP081477
• Coordinates: 6698587..6699735 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain P8W
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6693587..6704735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5A80_RS31845 (K5A80_32590)	-	6694738..6695034 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
K5A80_RS31850 (K5A80_32595)	-	6695031..6695624 (-)	594	WP_003084544.1	YggT family protein	-
K5A80_RS31855 (K5A80_32600)	proC	6695635..6696456 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
K5A80_RS31860 (K5A80_32605)	-	6696468..6697160 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
K5A80_RS31865 (K5A80_32610)	pilT	6697375..6698409 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
K5A80_RS31870 (K5A80_32615)	pilU	6698587..6699735 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
K5A80_RS31875 (K5A80_32620)	czcD	6699743..6700642 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
K5A80_RS31880 (K5A80_32625)	-	6700796..6701200 (+)	405	WP_003084560.1	NINE protein	-
K5A80_RS31885 (K5A80_32630)	-	6701443..6702816 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
K5A80_RS31890 (K5A80_32635)	-	6702813..6703997 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=596753 K5A80_RS31870 WP_003110016.1 6698587..6699735(+) (pilU) [Pseudomonas aeruginosa strain P8W]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=596753 K5A80_RS31870 WP_003110016.1 6698587..6699735(+) (pilU) [Pseudomonas aeruginosa strain P8W]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382