Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   AW27_RS14340 Genome accession   NZ_CP097800
Coordinates   3342279..3343721 (+) Length   480 a.a.
NCBI ID   WP_037921243.1    Uniprot ID   -
Organism   Streptomyces sp. PCS3-D2 isolate P1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3337279..3348721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW27_RS14315 (AW27_014295) - 3337926..3338099 (+) 174 WP_157840233.1 hypothetical protein -
  AW27_RS14320 (AW27_014300) - 3338092..3338322 (+) 231 WP_037921251.1 hypothetical protein -
  AW27_RS14325 (AW27_014305) - 3338310..3339260 (-) 951 WP_037921249.1 Ppx/GppA phosphatase family protein -
  AW27_RS14330 (AW27_014310) - 3339324..3340211 (+) 888 WP_037921247.1 hypothetical protein -
  AW27_RS14335 (AW27_014315) - 3340330..3342096 (-) 1767 WP_037921246.1 sigma-70 family RNA polymerase sigma factor -
  AW27_RS14340 (AW27_014320) radA/sms 3342279..3343721 (+) 1443 WP_037921243.1 DNA repair protein RadA Machinery gene
  AW27_RS14345 (AW27_014325) disA 3344028..3345152 (+) 1125 WP_037921241.1 DNA integrity scanning diadenylate cyclase DisA Machinery gene
  AW27_RS14350 (AW27_014330) - 3345269..3346072 (-) 804 WP_037921239.1 hypothetical protein -
  AW27_RS14355 (AW27_014335) - 3346233..3346886 (+) 654 WP_106967579.1 phosphatase PAP2 family protein -
  AW27_RS14360 (AW27_014340) - 3346883..3347848 (+) 966 WP_037921238.1 A/G-specific adenine glycosylase -
  AW27_RS14365 (AW27_014345) - 3348123..3348689 (+) 567 WP_037921236.1 SigE family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 480 a.a.        Molecular weight: 50199.34 Da        Isoelectric Point: 8.2598

>NTDB_id=596513 AW27_RS14340 WP_037921243.1 3342279..3343721(+) (radA/sms) [Streptomyces sp. PCS3-D2 isolate P1]
MAARTSRSSAKDRPSYRCTECGWTTAKWLGRCPECQAWGTVEEMGAPAVRTTAAGRVSTAALPIAQVDGRTATARSTGVD
ELDRVLGGGLVPGAVVLLAGEPGVGKSTLLLDVAAKASSDTHRTLYVTGEESASQVRLRADRINALSDHLYLAAETDLSA
VLGHLDAVQPSLLVLDSVQTVASPEIDGAPGGMAQVREVAGALIRASKERGMATLLVGHVTKDGAIAGPRLLEHLVDVVL
SFEGDRHARLRLVRGVKNRYGATDEVGCFELHDEGITGLADPSGLFLTRRAEAVPGTCLTVTLEGKRPLVAEVQALTVDS
QIPSPRRTTSGLETSRVSMMLAVLEQRGRITALGKRDIYSATVGGVKLTEPAADLAIALALASAASDVPLPKNLVAIGEV
GLAGEVRRVTGVQRRLAEAHRLGFTHALVPTDPGKVPPGMKVTEVADMGDALRVLPRGRSRTAARESKESSERAGAGAAR

Nucleotide


Download         Length: 1443 bp        

>NTDB_id=596513 AW27_RS14340 WP_037921243.1 3342279..3343721(+) (radA/sms) [Streptomyces sp. PCS3-D2 isolate P1]
ATGGCTGCCCGCACCTCTCGCTCATCCGCCAAGGACCGACCGTCCTACCGCTGCACCGAGTGCGGCTGGACGACGGCCAA
GTGGCTCGGCCGGTGTCCCGAGTGCCAGGCCTGGGGCACCGTCGAGGAGATGGGGGCACCCGCCGTACGGACCACCGCGG
CCGGCCGGGTGTCGACCGCCGCCCTGCCGATCGCCCAGGTCGACGGGCGGACGGCGACCGCCCGCAGCACCGGTGTGGAC
GAGCTGGACCGCGTCCTGGGCGGCGGGCTCGTGCCCGGCGCCGTCGTCCTGCTGGCCGGCGAGCCCGGCGTGGGCAAGTC
CACGCTGCTGCTGGACGTCGCGGCGAAGGCCTCCAGCGACACGCACCGCACGCTGTACGTGACGGGTGAGGAATCGGCGA
GCCAGGTCCGGTTGCGGGCGGACCGCATCAACGCGCTGAGCGACCACCTGTACCTCGCGGCCGAGACCGACCTGTCCGCG
GTGCTCGGGCACCTCGACGCCGTGCAGCCCTCCCTGCTCGTCCTGGACTCCGTACAGACCGTCGCCTCCCCCGAGATCGA
CGGCGCGCCCGGCGGCATGGCCCAGGTGCGGGAGGTGGCGGGCGCGCTGATCCGGGCCTCCAAGGAGCGGGGCATGGCCA
CCCTGCTCGTCGGCCACGTCACCAAGGACGGGGCCATCGCCGGTCCCCGGCTCCTGGAACACCTCGTCGACGTGGTGCTG
AGTTTCGAGGGAGACCGGCACGCGCGGCTACGGCTCGTGCGCGGCGTGAAGAACCGGTACGGCGCCACCGACGAGGTCGG
CTGCTTCGAACTGCACGACGAGGGCATCACCGGGCTGGCCGACCCGAGCGGGCTGTTCCTGACCCGGCGTGCCGAGGCCG
TCCCGGGAACGTGCCTGACCGTCACCCTGGAAGGGAAGCGGCCACTGGTCGCCGAGGTGCAGGCGCTGACCGTCGACTCG
CAGATCCCCTCCCCCCGGCGGACCACCTCGGGCCTGGAGACCTCGCGCGTCTCGATGATGCTGGCGGTGCTGGAGCAGCG
CGGCAGGATCACCGCGCTCGGCAAACGCGACATCTACAGCGCCACCGTGGGCGGGGTGAAGCTCACCGAACCGGCCGCGG
ACCTGGCGATCGCGCTCGCGCTGGCGTCCGCCGCCAGCGACGTCCCGCTCCCGAAGAACCTGGTGGCCATCGGCGAGGTC
GGCCTGGCCGGCGAGGTGCGGCGGGTGACCGGCGTACAGCGGCGGCTGGCGGAAGCGCACCGGCTGGGCTTCACCCACGC
GCTGGTGCCGACCGACCCGGGCAAGGTGCCACCGGGGATGAAGGTCACCGAAGTGGCCGACATGGGCGACGCGCTGCGGG
TGCTGCCGCGCGGGCGCTCCCGTACGGCGGCCAGGGAGAGCAAGGAGAGCAGCGAGCGGGCGGGGGCCGGGGCCGCGAGG
TAG

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(16-43)

Subunit ChlI of Mg-chelatase (ChlI)

(372-431)

AAA domain (AAA_25)

(84-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

44.371

94.375

0.419
  radA Streptococcus mitis NCTC 12261

43.584

94.167

0.41
  radA Streptococcus mitis SK321

44.495

90.833

0.404
  radA Streptococcus pneumoniae TIGR4

44.626

89.167

0.398
  radA Streptococcus pneumoniae D39

44.626

89.167

0.398
  radA Streptococcus pneumoniae Rx1

44.626

89.167

0.398
  radA Streptococcus pneumoniae R6

44.626

89.167

0.398