Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   M6G44_RS06550 Genome accession   NZ_CP097377
Coordinates   1432263..1432811 (-) Length   182 a.a.
NCBI ID   WP_005617759.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain GD2107     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1427263..1437811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6G44_RS06535 (M6G44_06535) - 1427301..1428587 (+) 1287 WP_005617755.1 NAD(P)/FAD-dependent oxidoreductase -
  M6G44_RS06540 (M6G44_06540) - 1428777..1431116 (-) 2340 WP_005617757.1 Tex family protein -
  M6G44_RS06545 (M6G44_06545) - 1431165..1432139 (-) 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
  M6G44_RS06550 (M6G44_06550) pilF 1432263..1432811 (-) 549 WP_005617759.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  M6G44_RS06555 (M6G44_06555) - 1432865..1434046 (-) 1182 WP_005608495.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  M6G44_RS06580 (M6G44_06580) gltX 1434951..1436390 (+) 1440 WP_005605060.1 glutamate--tRNA ligase -
  M6G44_RS06590 (M6G44_06590) yefM 1436728..1436979 (+) 252 WP_017357689.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  M6G44_RS06595 (M6G44_06595) - 1436976..1437236 (+) 261 WP_017357690.1 Txe/YoeB family addiction module toxin -
  M6G44_RS06600 (M6G44_06600) - 1437395..1437562 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20645.27 Da        Isoelectric Point: 6.9479

>NTDB_id=594803 M6G44_RS06550 WP_005617759.1 1432263..1432811(-) (pilF) [Actinobacillus pleuropneumoniae strain GD2107]
MNLAKFLRNLTACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKVNEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=594803 M6G44_RS06550 WP_005617759.1 1432263..1432811(-) (pilF) [Actinobacillus pleuropneumoniae strain GD2107]
ATGAACCTTGCAAAATTTTTGCGAAATTTGACCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
TACCTCTCAAACCACGTTTAATCGCTCGGAAGCGGTTAAAGCGCGTATCAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCATGATCAAAACGACTACTTACCTCACTCCGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATCGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGTAAACGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

66.489

100

0.687
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.199

93.956

0.434