Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   M4R69_RS21525 Genome accession   NZ_CP097198
Coordinates   4452188..4452997 (+) Length   269 a.a.
NCBI ID   WP_001697419.1    Uniprot ID   -
Organism   Escherichia coli strain 2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4447188..4457997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M4R69_RS21520 (M4R69_21515) sslE 4447493..4452058 (+) 4566 WP_181201690.1 lipoprotein metalloprotease SslE -
  M4R69_RS21525 (M4R69_21520) pilD 4452188..4452997 (+) 810 WP_001697419.1 prepilin peptidase PppA Machinery gene
  M4R69_RS21530 (M4R69_21525) gspS2 4453063..4453473 (+) 411 WP_001361238.1 type II secretion system pilot lipoprotein GspS-beta -
  M4R69_RS21535 (M4R69_21530) gspC 4453491..4454450 (+) 960 WP_000135085.1 type II secretion system protein GspC -
  M4R69_RS21540 (M4R69_21535) gspD 4454480..4456540 (+) 2061 WP_001468189.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29429.08 Da        Isoelectric Point: 8.3801

>NTDB_id=593968 M4R69_RS21525 WP_001697419.1 4452188..4452997(+) (pilD) [Escherichia coli strain 2-1]
MFFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGPLSLPNVALIASCCGLIYAV
ITKRGSTTLPFGPCLSLGGIATLYLQALL

Nucleotide


Download         Length: 810 bp        

>NTDB_id=593968 M4R69_RS21525 WP_001697419.1 4452188..4452997(+) (pilD) [Escherichia coli strain 2-1]
ATGTTTTTTGATGTTTTTCAGCAATACCCCGCGGCGATGCCCGTCCTGGCTACCGTCGGAGGATTGATTATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCACGGTGAAATGCCGAGTGCGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGCCTGGTCTGGCCAGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATAACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACT
GGGCTGATTGCGGCATGGGCGCAACAGAGTCCCTTAACGCTACAAGACGCAGTTACCGGCGTACTGGTGGGGTTTATCAC
TTTTTACTCTCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTTTTCGCTG
CGTTAGGTGGCTGGGTGGGGCCGTTGTCGCTACCCAATGTTGCTTTAATCGCATCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAGAGGATCAACCACACTGCCTTTTGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGCTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

42.435

100

0.428
  pilD Neisseria gonorrhoeae MS11

39.3

95.539

0.375
  pilD Acinetobacter nosocomialis M2

38.113

98.513

0.375
  pilD Acinetobacter baumannii D1279779

37.736

98.513

0.372