Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   Sta3580_RS10370 Genome accession   NZ_CP080458
Coordinates   2135730..2136704 (-) Length   324 a.a.
NCBI ID   WP_015900415.1    Uniprot ID   B9DNP2
Organism   Staphylococcus carnosus strain KCTC 3580     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2130730..2141704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Sta3580_RS10335 (Sta3580_10295) gcvT 2131221..2132315 (-) 1095 WP_015900410.1 glycine cleavage system aminomethyltransferase GcvT -
  Sta3580_RS10340 (Sta3580_10300) - 2132506..2133045 (-) 540 WP_015900411.1 shikimate kinase -
  Sta3580_RS10345 (Sta3580_10305) comGF 2133216..2133674 (-) 459 WP_158297486.1 competence type IV pilus minor pilin ComGF -
  Sta3580_RS10350 (Sta3580_10310) - 2133631..2133927 (-) 297 WP_041612977.1 hypothetical protein -
  Sta3580_RS10355 (Sta3580_10315) - 2133908..2134321 (-) 414 WP_231635362.1 prepilin-type cleavage/methylation domain-containing protein -
  Sta3580_RS10360 (Sta3580_10320) comGC 2134341..2134667 (-) 327 WP_015900413.1 competence type IV pilus major pilin ComGC Machinery gene
  Sta3580_RS10365 (Sta3580_10325) comGB 2134685..2135758 (-) 1074 WP_015900414.1 competence type IV pilus assembly protein ComGB -
  Sta3580_RS10370 (Sta3580_10330) comGA 2135730..2136704 (-) 975 WP_015900415.1 competence type IV pilus ATPase ComGA Machinery gene
  Sta3580_RS10375 (Sta3580_10335) - 2136775..2137398 (-) 624 WP_015900416.1 MBL fold metallo-hydrolase -
  Sta3580_RS10380 (Sta3580_10340) - 2137398..2137727 (-) 330 WP_015900417.1 MTH1187 family thiamine-binding protein -
  Sta3580_RS10385 (Sta3580_10345) - 2137727..2138713 (-) 987 WP_015900418.1 ROK family glucokinase -
  Sta3580_RS10390 (Sta3580_10350) - 2138713..2138913 (-) 201 WP_015900419.1 YqgQ family protein -
  Sta3580_RS10395 (Sta3580_10355) - 2138894..2140354 (-) 1461 WP_015900420.1 rhomboid family intramembrane serine protease -
  Sta3580_RS10400 (Sta3580_10360) - 2140371..2140913 (-) 543 WP_015900421.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36963.71 Da        Isoelectric Point: 7.1254

>NTDB_id=593010 Sta3580_RS10370 WP_015900415.1 2135730..2136704(-) (comGA) [Staphylococcus carnosus strain KCTC 3580]
MKRLLDTILTDAISKNVSDIHFIPIEKEVIIKFRIHDDLINYDNIEILTYSKLLTFMKFQAGLDVSNYHQAQSGQTTFKH
QKLYNLRVSTLPLSLGIESCVIRLSPQYFYQDTDRDGPKHFYHLMNKKQGLILFTGPTGSGKSTMMYEMVSFALKNLHLN
IITVEDPVEKRIAGITQISVNEKAGIDYENSFKAILRCDPDIILIGEIRDAQIAKCVIQASLSGHLVLSTLHSNDCEGAL
LRLLEMGISPQEAQQSIALISNQRLVTNQHGSRELAYETMYQSDIQYFFKHNHTMPKHFMKLSKVLTQMSEEGVICEEVL
KKYT

Nucleotide


Download         Length: 975 bp        

>NTDB_id=593010 Sta3580_RS10370 WP_015900415.1 2135730..2136704(-) (comGA) [Staphylococcus carnosus strain KCTC 3580]
ATGAAAAGACTTTTAGATACAATTCTCACAGATGCAATTTCGAAAAATGTATCTGATATCCATTTTATTCCAATTGAAAA
AGAAGTTATAATCAAGTTCAGAATTCACGATGATTTAATTAATTATGACAATATCGAAATTCTGACTTATTCAAAGTTAC
TTACGTTTATGAAATTTCAAGCAGGGCTCGATGTATCTAATTATCATCAAGCACAAAGTGGTCAGACAACTTTCAAGCAT
CAAAAACTATATAATTTACGTGTTTCTACTTTACCGTTATCCTTAGGGATTGAGAGTTGTGTAATACGACTTTCACCCCA
GTATTTTTATCAAGATACCGATCGTGATGGTCCTAAACACTTCTATCATTTAATGAATAAAAAGCAAGGACTTATTTTAT
TCACGGGACCAACCGGTTCTGGAAAAAGTACTATGATGTATGAAATGGTTTCTTTTGCTTTAAAAAATTTGCATCTAAAT
ATTATTACTGTTGAAGACCCAGTTGAGAAAAGAATTGCTGGCATTACTCAAATTTCAGTAAATGAGAAAGCAGGTATAGA
TTATGAAAATTCATTTAAAGCTATATTACGCTGTGATCCCGATATTATACTTATTGGGGAGATAAGAGATGCTCAAATCG
CAAAATGTGTTATTCAAGCATCTCTAAGCGGACATCTAGTGCTTTCCACTTTGCATTCAAATGATTGCGAAGGAGCATTA
TTACGACTCTTGGAAATGGGTATATCTCCACAAGAAGCACAACAATCCATTGCTCTAATTTCAAATCAACGTCTAGTAAC
TAATCAACATGGAAGTAGAGAACTAGCTTATGAAACGATGTATCAATCTGATATTCAATACTTCTTCAAACATAACCATA
CAATGCCTAAACATTTTATGAAATTAAGCAAAGTACTAACTCAAATGAGTGAAGAAGGTGTTATATGTGAAGAAGTACTT
AAAAAATATACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9DNP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

60.681

99.691

0.605
  comGA Staphylococcus aureus N315

60.681

99.691

0.605