Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L483_RS29990 Genome accession   NC_021491
Coordinates   6478134..6479396 (-) Length   420 a.a.
NCBI ID   WP_016489623.1    Uniprot ID   -
Organism   Pseudomonas putida H8234     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6473134..6484396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L483_RS33700 - 6474443..6474667 (-) 225 WP_132844974.1 hypothetical protein -
  L483_RS29975 (L483_30530) - 6474797..6476401 (-) 1605 WP_016489620.1 SPOR domain-containing protein -
  L483_RS29980 (L483_30535) aroB 6476417..6477514 (-) 1098 WP_024717572.1 3-dehydroquinate synthase -
  L483_RS29985 (L483_30540) aroK 6477611..6478129 (-) 519 WP_024717571.1 shikimate kinase AroK -
  L483_RS29990 (L483_30545) pilQ 6478134..6479396 (-) 1263 WP_016489623.1 type IV pilus secretin PilQ Machinery gene
  L483_RS29995 (L483_30550) - 6479320..6480336 (-) 1017 WP_016489624.1 pilus assembly protein PilP -
  L483_RS30000 (L483_30555) - 6480333..6480869 (-) 537 WP_016489625.1 PilN domain-containing protein -
  L483_RS30005 (L483_30560) pilM 6480866..6481747 (-) 882 WP_016489626.1 type IV pilus biogenesis protein PilM -
  L483_RS30010 (L483_30565) - 6481942..6484392 (+) 2451 WP_371918298.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45918.61 Da        Isoelectric Point: 4.9055

>NTDB_id=59162 L483_RS29990 WP_016489623.1 6478134..6479396(-) (pilQ) [Pseudomonas putida H8234]
MKNVMKWVLAALLPIPLVWATPYQGEPLSLNFQDVEVRSVLQVLADYAGINLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKGLAQRHEGNVLLVAPAAAFAPQPFDAHPVPDAQMQPLRRELIPIHHAKAAELVELLLTSLADDSTFTVRGSLGVDERT
NTLVAHQPADRLAELRQLVTQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGESLRPGKELFVDLGVERAGSSVGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVAFREASLSLEVTPQITPDNKV
IMAVRVTKDEPDYVNAVNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDVLQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=59162 L483_RS29990 WP_016489623.1 6478134..6479396(-) (pilQ) [Pseudomonas putida H8234]
ATGAAGAACGTGATGAAATGGGTGTTGGCGGCCCTGCTGCCGATCCCGCTGGTGTGGGCGACGCCGTATCAGGGTGAGCC
ACTTTCTTTGAATTTCCAGGATGTAGAGGTTCGCTCGGTGCTGCAGGTGCTGGCCGATTACGCGGGCATCAATCTGGTTG
CCAGCGATGACGTGCAGGGCAACGTGACCTTGCGTTTGCAGGACGTGCCCTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGGCACAGCGGCACGAAGGCAATGTGTTGCTGGTGGCCCCTGCGGCAGCGTTTGCCCCGCAGCCGTTCGA
TGCCCATCCGGTGCCGGATGCGCAGATGCAGCCGTTACGCCGTGAACTGATACCGATCCATCATGCCAAGGCGGCGGAGC
TGGTTGAGTTGTTGCTGACCAGCCTGGCGGATGACAGCACCTTCACCGTTCGTGGCAGCCTGGGTGTCGATGAGCGCACC
AATACCCTGGTGGCGCATCAGCCGGCCGATCGCCTGGCCGAATTGCGGCAACTGGTTACGCAGCTGGACGTGCCGGTGCG
CCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGACTACGAGAAAAGCCTGGGGGTGCGCTGGGGCGGGCCGC
TGTACGGGGAAAGCCTGCGCCCGGGCAAGGAACTGTTCGTCGACCTCGGCGTGGAGCGGGCGGGCAGCAGCGTCGGCCTG
GGCCTGCTGCGCGGTGACGTGTTGCTCGACCTGGAACTCAGCGCCATGGAAAAAAGCGGCAACGGCGAGATCATCTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCAGCCAGA
GTGGCGCCACCTCGGTCGCCTTCCGCGAAGCCTCGCTTTCGCTGGAGGTGACCCCGCAGATTACACCTGACAACAAGGTA
ATCATGGCTGTCAGGGTGACCAAGGACGAGCCTGATTACGTCAATGCCGTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTTGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Bacterial type II/III secretion system short domain (Secretin_N)

(123-188)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.998

100

0.617
  pilQ Acinetobacter baumannii strain A118

42.241

100

0.467
  pilQ Acinetobacter baumannii D1279779

42.241

100

0.467
  comQ Acinetobacter baylyi ADP1

43.111

100

0.462
  pilQ Legionella pneumophila strain Lp02

42.143

100

0.421
  pilQ Legionella pneumophila strain ERS1305867

42.143

100

0.421
  pilQ Vibrio campbellii strain DS40M4

40.941

100

0.414
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.487

99.286

0.412
  pilQ Vibrio cholerae strain A1552

41.487

99.286

0.412

Multiple sequence alignment