Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ITG12_RS12745 Genome accession   NZ_CP080238
Coordinates   2743344..2744360 (+) Length   338 a.a.
NCBI ID   WP_248873119.1    Uniprot ID   -
Organism   Vibrio sp. ED002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2738344..2749360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG12_RS12730 (ITG12_12650) - 2738719..2739447 (-) 729 WP_038865508.1 redoxin family protein -
  ITG12_RS12735 (ITG12_12655) oxyR 2739623..2740531 (+) 909 WP_038865506.1 DNA-binding transcriptional regulator OxyR -
  ITG12_RS12740 (ITG12_12660) - 2740601..2743165 (-) 2565 WP_038881654.1 PBP1A family penicillin-binding protein -
  ITG12_RS12745 (ITG12_12665) pilM 2743344..2744360 (+) 1017 WP_248873119.1 type IV pilus assembly protein PilM Machinery gene
  ITG12_RS12750 (ITG12_12670) pilN 2744344..2744910 (+) 567 WP_038865500.1 PilN domain-containing protein Machinery gene
  ITG12_RS12755 (ITG12_12675) pilO 2744903..2745496 (+) 594 WP_038865498.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG12_RS12760 (ITG12_12680) pilP 2745486..2746001 (+) 516 WP_038865496.1 pilus assembly protein PilP Machinery gene
  ITG12_RS12765 (ITG12_12685) pilQ 2746036..2747787 (+) 1752 WP_045405855.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG12_RS12770 (ITG12_12690) aroK 2747976..2748494 (+) 519 WP_038865492.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37899.29 Da        Isoelectric Point: 6.4321

>NTDB_id=591571 ITG12_RS12745 WP_248873119.1 2743344..2744360(+) (pilM) [Vibrio sp. ED002]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKDLPFRANRVAISVPDNA
VISKKLQIEQSLEESEKEFAVVQAFSHQSPFPVEELSLDFVLVESDEQMRGTESYQVFATRKDVVETRVEALQKSGLKTV
LVDVHSQSLGHIWKLTTERYPDKSKYCLLDIGVLNSSFTMFTEQGELFHKEFVCGTRAASGLLHDNPLSDDAQVMTEQFN
RQVVDRVKRQMQLYTSINGSQDIKGIWLSGEGASTPMLAEELSSQLALECELFNPLGLFEMKVSKRKRRAADWQHFTTAA
GLAVRGIQWLGGHHALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=591571 ITG12_RS12745 WP_248873119.1 2743344..2744360(+) (pilM) [Vibrio sp. ED002]
ATGGATAAGCTATTAGTTACGGGCATAGATATCGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTATGCCTTACTGGGTTACAAAGAAATAATACTTAAAGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTAAAAGAGATGAAAAAAGACCTCCCTTTTAGGGCTAATCGAGTCGCGATTTCAGTTCCTGACAATGCG
GTGATCAGCAAAAAACTGCAAATCGAACAAAGTTTAGAGGAAAGTGAAAAAGAGTTTGCCGTCGTACAGGCTTTTTCTCA
TCAATCGCCGTTCCCTGTAGAAGAGCTTAGTCTTGATTTTGTATTAGTGGAATCTGATGAGCAAATGCGTGGGACAGAAA
GCTATCAAGTTTTCGCAACACGTAAAGACGTGGTGGAAACCCGCGTAGAAGCATTGCAAAAATCTGGTCTAAAAACCGTG
CTGGTGGACGTGCATTCCCAAAGTCTTGGGCATATCTGGAAGTTAACCACGGAACGCTACCCTGACAAAAGCAAATACTG
CCTACTGGACATCGGGGTATTAAATAGCTCGTTCACTATGTTTACCGAGCAAGGTGAGTTATTCCATAAAGAGTTTGTGT
GTGGCACTCGTGCCGCTAGTGGCCTTTTGCACGACAACCCGCTTTCTGATGATGCTCAAGTCATGACAGAACAATTCAAC
CGTCAAGTTGTGGATCGCGTGAAACGTCAAATGCAACTCTATACCTCTATTAATGGTTCTCAAGATATTAAAGGTATTTG
GCTCTCCGGTGAGGGGGCGTCCACCCCAATGTTAGCTGAAGAGCTCTCCAGTCAACTGGCGCTCGAATGTGAGTTGTTTA
ACCCACTGGGCTTATTTGAAATGAAAGTTTCTAAGCGCAAGCGCCGCGCCGCAGATTGGCAGCACTTCACTACCGCTGCG
GGTTTGGCAGTTCGTGGTATCCAGTGGCTGGGAGGTCATCATGCTCTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

88.166

100

0.882
  pilM Vibrio cholerae strain A1552

49.123

100

0.497