Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ITG12_RS12755 Genome accession   NZ_CP080238
Coordinates   2744903..2745496 (+) Length   197 a.a.
NCBI ID   WP_038865498.1    Uniprot ID   A0A2N7MTC5
Organism   Vibrio sp. ED002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2739903..2750496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG12_RS12740 (ITG12_12660) - 2740601..2743165 (-) 2565 WP_038881654.1 PBP1A family penicillin-binding protein -
  ITG12_RS12745 (ITG12_12665) pilM 2743344..2744360 (+) 1017 WP_248873119.1 type IV pilus assembly protein PilM Machinery gene
  ITG12_RS12750 (ITG12_12670) pilN 2744344..2744910 (+) 567 WP_038865500.1 PilN domain-containing protein Machinery gene
  ITG12_RS12755 (ITG12_12675) pilO 2744903..2745496 (+) 594 WP_038865498.1 type 4a pilus biogenesis protein PilO Machinery gene
  ITG12_RS12760 (ITG12_12680) pilP 2745486..2746001 (+) 516 WP_038865496.1 pilus assembly protein PilP Machinery gene
  ITG12_RS12765 (ITG12_12685) pilQ 2746036..2747787 (+) 1752 WP_045405855.1 type IV pilus secretin PilQ family protein Machinery gene
  ITG12_RS12770 (ITG12_12690) aroK 2747976..2748494 (+) 519 WP_038865492.1 shikimate kinase AroK -
  ITG12_RS12775 (ITG12_12695) aroB 2748526..2749626 (+) 1101 WP_248873120.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23072.67 Da        Isoelectric Point: 5.1105

>NTDB_id=591573 ITG12_RS12755 WP_038865498.1 2744903..2745496(+) (pilO) [Vibrio sp. ED002]
MVNWQDLDMDEIAEWPVLPQLVVILLMVLLIQGFGYWFYLKPEEERLNTLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGKKQSEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEEQKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=591573 ITG12_RS12755 WP_038865498.1 2744903..2745496(+) (pilO) [Vibrio sp. ED002]
GTGGTTAATTGGCAAGATCTTGATATGGATGAAATTGCTGAGTGGCCAGTGCTGCCTCAGTTAGTGGTCATCTTATTAAT
GGTGCTTTTAATCCAAGGTTTTGGGTATTGGTTTTACTTAAAGCCCGAAGAGGAGCGCTTGAATACGTTGATTCAAGAAG
AGCAAACCTTGAAAGTCGCGTTGCGCATCAAAGCTAACAAAGTTGCGGCCTTACCCCAGCTACAATCTCAACTTGATGAG
TTAACCACGCGCTACGAGTTGTTGCTTCAGCAATTACCTGCGCAAAAAGAACTGGCAACTATGTTGGCCTCAGTCAACGA
ACTCGGTATTGATAACAAACTGACTTTTACTCGGATTGACTGGGGTAAAAAACAATCTGAACACTTCCTTTATCGGTTAC
CGCTCAATATCGAGTTAACGGGAGACTTCCACGACATTGGTCGTTTTTCACAAGCGATAGCGACATTACCTCGCATCATC
ACATTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAGACGTTGCACTTCCGTGTTAAGGCGAATACATACCA
GTTCAAACCAGAGGAACAGAAAGATGCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7MTC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

71.728

96.954

0.695