Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M1Y13_RS20390 Genome accession   NZ_CP096902
Coordinates   4133459..4133899 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain RSHH22     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4128459..4138899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1Y13_RS20365 (M1Y13_20370) coaE 4128949..4129569 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  M1Y13_RS20370 (M1Y13_20375) - 4129594..4129638 (+) 45 WP_120795372.1 protein YacM -
  M1Y13_RS20375 (M1Y13_20380) guaC 4129794..4130837 (+) 1044 WP_001217338.1 GMP reductase -
  M1Y13_RS20380 (M1Y13_20385) hofC 4130872..4132074 (-) 1203 WP_000157234.1 protein transport protein HofC -
  M1Y13_RS20385 (M1Y13_20390) pilB 4132064..4133449 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  M1Y13_RS20390 (M1Y13_20395) pilA 4133459..4133899 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  M1Y13_RS20395 (M1Y13_20400) nadC 4134102..4134995 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M1Y13_RS20400 (M1Y13_20405) ampD 4135083..4135634 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M1Y13_RS20405 (M1Y13_20410) ampE 4135631..4136485 (+) 855 WP_129541682.1 beta-lactamase regulator AmpE -
  M1Y13_RS20410 (M1Y13_20415) aroP 4136528..4137901 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=591135 M1Y13_RS20390 WP_000360902.1 4133459..4133899(-) (pilA) [Escherichia coli strain RSHH22]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=591135 M1Y13_RS20390 WP_000360902.1 4133459..4133899(-) (pilA) [Escherichia coli strain RSHH22]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377