Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KYI08_RS07295 Genome accession   NZ_CP079950
Coordinates   1395467..1396438 (-) Length   323 a.a.
NCBI ID   WP_012657092.1    Uniprot ID   B9E6S6
Organism   Macrococcoides caseolyticum strain 19Msa0287     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1390467..1401438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYI08_RS07260 (KYI08_07260) gcvT 1391164..1392249 (-) 1086 WP_041636415.1 glycine cleavage system aminomethyltransferase GcvT -
  KYI08_RS07265 (KYI08_07265) - 1392387..1392893 (-) 507 WP_101057095.1 shikimate kinase -
  KYI08_RS07270 (KYI08_07270) comGF 1393054..1393506 (-) 453 WP_086038773.1 competence type IV pilus minor pilin ComGF -
  KYI08_RS07275 (KYI08_07275) - 1393451..1393693 (-) 243 WP_157819992.1 hypothetical protein -
  KYI08_RS07280 (KYI08_07280) comGD 1393680..1394105 (-) 426 WP_101037039.1 competence type IV pilus minor pilin ComGD -
  KYI08_RS07285 (KYI08_07285) comGC 1394098..1394421 (-) 324 WP_101037082.1 competence type IV pilus major pilin ComGC Machinery gene
  KYI08_RS07290 (KYI08_07290) comGB 1394443..1395516 (-) 1074 WP_086038845.1 competence type IV pilus assembly protein ComGB -
  KYI08_RS07295 (KYI08_07295) comGA 1395467..1396438 (-) 972 WP_012657092.1 competence type IV pilus ATPase ComGA Machinery gene
  KYI08_RS07300 (KYI08_07300) - 1396650..1396898 (+) 249 WP_041636023.1 DUF2626 domain-containing protein -
  KYI08_RS07305 (KYI08_07305) - 1396925..1397545 (-) 621 WP_086038847.1 MBL fold metallo-hydrolase -
  KYI08_RS07310 (KYI08_07310) - 1397635..1397874 (+) 240 WP_041636024.1 DUF2759 domain-containing protein -
  KYI08_RS07315 (KYI08_07315) - 1397901..1398212 (-) 312 WP_086038848.1 MTH1187 family thiamine-binding protein -
  KYI08_RS07320 (KYI08_07320) - 1398212..1399180 (-) 969 WP_086038849.1 ROK family glucokinase -
  KYI08_RS07325 (KYI08_07325) - 1399177..1399368 (-) 192 WP_041636025.1 YqgQ family protein -
  KYI08_RS07330 (KYI08_07330) - 1399373..1400800 (-) 1428 WP_197932706.1 rhomboid family protein -
  KYI08_RS07335 (KYI08_07335) - 1400797..1401351 (-) 555 WP_041636026.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37136.78 Da        Isoelectric Point: 6.0532

>NTDB_id=590512 KYI08_RS07295 WP_012657092.1 1395467..1396438(-) (comGA) [Macrococcoides caseolyticum strain 19Msa0287]
MEKLFNEIIEQAILQSASDIHFIPCDKNVSIKFRVQGDIEEYSDIDDILFKKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
DNLRYNIRASTLPRSLGDEACVLRIIRQSFIDEYQTDDQILFDQMKKSSGIIIISGPTGSGKSTLMYQLVHFAKDTLKRQ
VISIEDPVEQHLDGIIQVNVNEKAEITYQTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLIKKRDEKERILAYEFLTKKDIEKYLKNKHINYRTLADILKEMYETNQISQHEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=590512 KYI08_RS07295 WP_012657092.1 1395467..1396438(-) (comGA) [Macrococcoides caseolyticum strain 19Msa0287]
ATGGAGAAATTATTCAATGAAATAATAGAGCAGGCAATTTTACAAAGTGCATCAGATATACACTTCATTCCTTGTGATAA
AAATGTATCTATTAAATTTAGGGTACAAGGTGATATCGAAGAATATAGTGACATCGACGATATATTATTTAAAAAATTAC
TTTCATATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCTCAGAGTGGGATAATACATTTTAATCTG
GATAACTTACGATATAATATTCGCGCATCTACTTTACCTCGTTCATTAGGCGATGAAGCATGTGTATTGAGAATCATCAG
ACAAAGTTTTATAGATGAATATCAGACAGATGATCAGATATTGTTCGATCAGATGAAAAAATCAAGCGGTATAATTATTA
TTAGTGGGCCAACTGGAAGTGGTAAGAGTACATTAATGTATCAACTTGTACATTTTGCAAAGGACACATTGAAACGCCAA
GTAATTTCAATAGAAGATCCTGTGGAGCAGCATCTTGACGGTATCATACAAGTTAATGTTAACGAAAAAGCAGAAATAAC
ATATCAAACCGCAATTAAGGCAATCCTTAGATGTGATCCAGATATCATTATGCTAGGTGAAATAAGAGATTCGGTCGTAG
CACAGCAAGTTATCAATGCGGGGCTGAGTGGTCATTTAGTTTTAACGACATTACATGCAAATGATTGTATTGGCGCATTA
TTTCGGTTAAAAGAAATGGGAATTAATGCTGTTGATCTTTATCAAAGTATCAATTTGATAATCAATCAAAGACTGATTAA
AAAAAGAGATGAAAAGGAGCGTATTCTAGCCTATGAATTTCTGACAAAAAAGGATATTGAAAAATATTTAAAGAATAAGC
ACATAAACTATAGAACGTTGGCTGACATACTAAAGGAGATGTATGAAACAAATCAGATTTCACAACATGAATTTGAAAAA
TTTGATCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E6S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.923

100

0.492
  comGA Staphylococcus aureus N315

48.923

100

0.492
  comGA Bacillus subtilis subsp. subtilis str. 168

34.77

100

0.375