Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BRPE64_RS01225 Genome accession   NC_021287
Coordinates   250285..251802 (-) Length   505 a.a.
NCBI ID   WP_044041062.1    Uniprot ID   -
Organism   Caballeronia insecticola     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245285..256802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRPE64_RS01200 (BRPE64_ACDS02480) - 246030..246662 (-) 633 WP_016344169.1 LON peptidase substrate-binding domain-containing protein -
  BRPE64_RS01205 (BRPE64_ACDS02490) rapZ 246697..247593 (-) 897 WP_016344170.1 RNase adapter RapZ -
  BRPE64_RS01210 (BRPE64_ACDS02500) hprK 247667..248635 (-) 969 WP_008351692.1 HPr(Ser) kinase/phosphatase -
  BRPE64_RS01215 (BRPE64_ACDS02510) ptsN 248822..249277 (-) 456 WP_044041872.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BRPE64_RS33125 (BRPE64_ACDS02520) - 249500..249667 (-) 168 WP_016344172.1 hypothetical protein -
  BRPE64_RS01220 (BRPE64_ACDS02530) hpf 249740..250099 (-) 360 WP_016344173.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BRPE64_RS01225 (BRPE64_ACDS02540) rpoN/rpoN1 250285..251802 (-) 1518 WP_044041062.1 RNA polymerase factor sigma-54 Machinery gene
  BRPE64_RS01230 (BRPE64_ACDS02550) lptB 251966..252733 (-) 768 WP_144063301.1 LPS export ABC transporter ATP-binding protein -
  BRPE64_RS01235 (BRPE64_ACDS02560) lptA 252745..253440 (-) 696 WP_016344176.1 lipopolysaccharide transport periplasmic protein LptA -
  BRPE64_RS01240 (BRPE64_ACDS02570) lptC 253471..254073 (-) 603 WP_016344177.1 LPS export ABC transporter periplasmic protein LptC -
  BRPE64_RS01245 (BRPE64_ACDS02580) - 254076..254639 (-) 564 WP_016344178.1 HAD family hydrolase -
  BRPE64_RS01250 (BRPE64_ACDS02590) kdsD 254639..255622 (-) 984 WP_016344179.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55447.44 Da        Isoelectric Point: 4.8796

>NTDB_id=58852 BRPE64_RS01225 WP_044041062.1 250285..251802(-) (rpoN/rpoN1) [Caballeronia insecticola]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWITSPLRVAADGSLITSPGASSAPPEPM
MSNGSSSSTSSSERSENSEPQGVDEYNGMSSDGGTDSSSWNLEDYGRSNNASDDDDLPPLQIHESTTTLRDHLTAQLRMT
SANQRDRALITFLIESLDEDGYLTSSLEEVQADLPDELEVDVDELSAALALLQSFDPPGVGGRSASECLKLQLLRLDASP
TRTLALDIVNNHLELLAARDFTRLRKQLKASDDSLRDAHVLIKSLEPFPGAAYGKSEADYVVPDILVRKSASGWTAELNP
EIVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKFFFGSHVSTDTGGAASSTAIRALIKQLIGAEEPKTPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=58852 BRPE64_RS01225 WP_044041062.1 250285..251802(-) (rpoN/rpoN1) [Caballeronia insecticola]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTACA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCTCAGAATCCGCTGCTGGAAAACGAGGACGACTGGA
TCACGAGTCCGCTGCGCGTCGCCGCGGACGGCTCGCTCATCACGTCGCCGGGGGCGTCCAGCGCGCCGCCCGAGCCGATG
ATGAGCAACGGGTCCTCGTCGAGCACGTCGAGTTCCGAACGTTCGGAAAACAGCGAGCCGCAAGGTGTCGACGAGTACAA
CGGCATGAGCTCGGACGGCGGCACGGATTCCAGTTCATGGAATCTTGAAGACTACGGCCGCTCAAACAACGCCTCCGACG
ACGACGATCTCCCCCCGCTCCAAATCCACGAATCGACCACAACGCTGCGCGATCATCTGACCGCGCAGTTGCGCATGACC
TCGGCGAACCAGCGCGATCGTGCGCTGATCACGTTTCTCATCGAATCGCTGGATGAAGACGGTTATCTGACGTCGTCGCT
CGAGGAAGTTCAGGCCGACTTGCCCGACGAACTGGAAGTGGACGTCGATGAACTGAGCGCCGCGCTCGCGCTGCTGCAAA
GCTTCGATCCACCGGGCGTGGGCGGCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAACTGCTGCGGCTCGATGCCTCGCCC
ACGCGCACGCTTGCGCTGGATATCGTCAACAATCATCTGGAATTACTGGCCGCGCGCGATTTCACGCGTTTGCGCAAGCA
ACTGAAGGCCAGCGACGACTCCCTGCGAGACGCGCATGTGCTGATCAAATCGCTCGAGCCGTTTCCCGGCGCGGCCTACG
GCAAGAGCGAAGCCGATTACGTGGTGCCGGACATTCTTGTGCGCAAGAGCGCCAGCGGATGGACGGCGGAGCTGAATCCG
GAGATCGTGCCGAAGCTGCGCATTAACCATTTGTACGCGAACATCCTGCGCAACAATCGCGGCGATCCGGGCAGCGGTTC
ACTGCGCCAGCAACTGCAAGAAGCACGCTGGTTGATCAAGAATATTCAGCAGAGATTCGAAACAATCCTGCGTGTTGCGC
AGGCCATTGTCGAGCGTCAAAAGAACTTTTTTGCACATGGCGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAAATTGCT
GATACGCTGGGTTTACACGAGTCTACGGTTTCGCGTGTAACCACGGGCAAGTACATGCTCACTCCATTCGGGACACTTGA
ATTTAAGTTCTTCTTTGGATCACACGTTTCAACCGACACGGGAGGCGCGGCATCGTCAACGGCGATCCGGGCCCTCATCA
AGCAACTGATAGGAGCGGAAGAGCCCAAAACTCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAGCAAGGATTC
GTGGTCGCGCGGCGTACTGTCGCCAAGTACCGCGAAGCCCTAAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.208

100

0.667

Multiple sequence alignment