Detailed information NTG0587691

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: KW113_RS02565
• Genome accession: NZ_CP079098
• Coordinates: 524838..525899 (+)
• Length: 353 a.a.
• NCBI ID: WP_017365267.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus strain IO1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 519838..530899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW113_RS02545 (KW113_02545)	-	520315..521088 (-)	774	WP_010959682.1	exodeoxyribonuclease III	-
KW113_RS02550 (KW113_02550)	rpmE	521203..521406 (+)	204	WP_010959683.1	50S ribosomal protein L31	-
KW113_RS02555 (KW113_02555)	-	521442..522053 (-)	612	WP_249385140.1	hypothetical protein	-
KW113_RS02560 (KW113_02560)	-	522069..524516 (-)	2448	WP_249385139.1	penicillin-binding protein 1A	-
KW113_RS02565 (KW113_02565)	pilM	524838..525899 (+)	1062	WP_017365267.1	pilus assembly protein PilM	Machinery gene
KW113_RS02570 (KW113_02570)	-	525899..526504 (+)	606	WP_017365266.1	PilN domain-containing protein	-
KW113_RS02575 (KW113_02575)	-	526501..527139 (+)	639	WP_010959688.1	type 4a pilus biogenesis protein PilO	-
KW113_RS02580 (KW113_02580)	-	527139..527681 (+)	543	WP_010959689.1	pilus assembly protein PilP	-
KW113_RS02585 (KW113_02585)	pilQ	527797..529857 (+)	2061	WP_256993206.1	type IV pilus secretin PilQ	-
KW113_RS02590 (KW113_02590)	aroK	529919..530458 (+)	540	WP_010959691.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38425.92 Da
• Isoelectric Point: 4.9350

>NTDB_id=587691 KW113_RS02565 WP_017365267.1 524838..525899(+) (pilM) [Methylococcus capsulatus strain IO1]
MWGLGRKKPLLLGIDISAAAVKLLELSRKNGGYQVESYGVVPLPRNTVVDNTLAEPDNVSAAVRAVVKQSGTALRHAVVA
VPGSVVITKRIALPANLEGDELEAQIELEADQYIPHAREEVSLDFEVLGKNPKNPDLLDVLLVATRRENVEDRVSVLENA
GLGVEIVDVESYAIERAFELVRDQLPPLLRDRAVAVADVGAMATTLNVIHGGSIIYTREQGFGGMQLTDEIQRRYGLTYE
EAGLAKKEGSLPGNYAEEVLEPFKHALVQQISRSFQFYLSSTAQRGFDAVVLAGGCAMIPGIDRYVEAALQVPTVIANPF
RHMSFSGRVRQERLSYDSSALMLACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=587691 KW113_RS02565 WP_017365267.1 524838..525899(+) (pilM) [Methylococcus capsulatus strain IO1]
ATGTGGGGCCTAGGAAGGAAGAAACCGCTCCTGCTGGGGATCGATATCAGCGCGGCGGCGGTCAAGCTGCTCGAGCTGAG
CAGAAAGAACGGCGGCTACCAGGTGGAAAGCTATGGCGTTGTGCCTTTGCCCCGCAACACGGTGGTCGACAACACCCTTG
CCGAGCCGGACAACGTGTCGGCGGCGGTCCGGGCCGTGGTCAAACAGTCGGGGACCGCGCTCAGGCACGCGGTGGTCGCG
GTACCCGGCTCGGTGGTCATCACCAAGCGCATCGCCCTACCCGCCAACCTGGAGGGCGACGAACTCGAGGCGCAGATCGA
ACTGGAGGCGGACCAGTACATTCCTCACGCCAGGGAGGAAGTCAGCCTGGATTTCGAGGTACTGGGGAAGAATCCGAAGA
ATCCCGATCTGCTGGATGTGCTGCTGGTGGCGACCCGCCGGGAGAACGTGGAAGACCGGGTCTCGGTACTGGAAAACGCC
GGACTCGGCGTCGAGATCGTCGATGTCGAGTCCTATGCCATCGAACGCGCCTTCGAACTTGTCCGGGATCAGCTTCCACC
GCTCCTCCGCGACCGGGCGGTTGCCGTAGCCGATGTGGGCGCCATGGCCACGACGCTCAACGTCATCCATGGCGGTTCCA
TCATCTACACGCGGGAACAGGGTTTCGGCGGCATGCAGCTCACCGACGAGATCCAGCGGCGCTACGGTTTGACCTACGAG
GAAGCGGGGCTGGCGAAGAAGGAGGGGAGCCTTCCCGGCAACTATGCCGAAGAGGTGCTGGAGCCGTTCAAGCATGCCTT
GGTTCAGCAGATCAGCCGATCGTTCCAGTTCTATCTGTCGTCCACTGCGCAGCGTGGTTTCGATGCGGTGGTGCTGGCCG
GCGGCTGCGCCATGATTCCGGGGATAGACCGTTACGTCGAGGCGGCGTTGCAGGTTCCCACCGTGATTGCCAATCCCTTC
CGTCACATGAGTTTTTCGGGAAGGGTGCGGCAGGAGCGCCTGAGTTACGATTCATCCGCCCTGATGCTGGCATGCGGCCT
GGCACTGCGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.603	97.167	0.501
comM	Acinetobacter nosocomialis M2	51.02	97.167	0.496
comM	Acinetobacter baylyi ADP1	49.138	98.584	0.484
pilM	Legionella pneumophila strain ERS1305867	42.733	97.45	0.416