Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KV188_RS03305 Genome accession   NZ_CP078508
Coordinates   723522..724070 (-) Length   182 a.a.
NCBI ID   WP_218633997.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae serovar 8 str. 405     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 718522..729070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KV188_RS03290 (KV188_03290) - 719016..719537 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  KV188_RS03295 (KV188_03295) - 719642..720928 (+) 1287 WP_005601799.1 NAD(P)/FAD-dependent oxidoreductase -
  KV188_RS03300 (KV188_03300) - 721120..723447 (-) 2328 WP_039709241.1 Tex family protein -
  KV188_RS03305 (KV188_03305) pilF 723522..724070 (-) 549 WP_218633997.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KV188_RS03310 (KV188_03310) - 724124..725305 (-) 1182 WP_005605050.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  KV188_RS03335 (KV188_03335) gltX 726210..727649 (+) 1440 WP_005608501.1 glutamate--tRNA ligase -
  KV188_RS03345 (KV188_03345) yefM 727987..728238 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  KV188_RS03350 (KV188_03350) - 728235..728495 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  KV188_RS03355 (KV188_03355) - 728654..728821 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20550.14 Da        Isoelectric Point: 6.6260

>NTDB_id=587514 KV188_RS03305 WP_218633997.1 723522..724070(-) (pilF) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNCPDVLNNYGTFLCKQAKFDQAYQQFEQAVKSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=587514 KV188_RS03305 WP_218633997.1 723522..724070(-) (pilF) [Actinobacillus pleuropneumoniae serovar 8 str. 405]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATTGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATATCAGCAGTTTGAGCAAGCGG
TGAAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.426

100

0.676
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44