Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KWY99_RS02170 Genome accession   NZ_CP078118
Coordinates   403479..403943 (-) Length   154 a.a.
NCBI ID   WP_167549147.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain MS11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 400023..421759 403479..403943 within 0


Gene organization within MGE regions


Location: 400023..421759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KWY99_RS02155 (KWY99_02155) - 400023..401237 (-) 1215 WP_003694614.1 replication initiation factor domain-containing protein -
  KWY99_RS02160 (KWY99_02160) - 401939..402304 (-) 366 Protein_404 pilin -
  KWY99_RS11790 - 402344..402745 (-) 402 Protein_405 pilin -
  KWY99_RS02165 (KWY99_02165) - 403133..403540 (-) 408 Protein_406 pilin -
  KWY99_RS02170 (KWY99_02170) pilE 403479..403943 (-) 465 WP_167549147.1 pilin Machinery gene
  KWY99_RS11795 - 403968..404312 (-) 345 Protein_408 pilin -
  KWY99_RS11800 - 404352..404573 (-) 222 Protein_409 pilin -
  KWY99_RS02180 (KWY99_02180) - 404938..405069 (-) 132 WP_010356054.1 hypothetical protein -
  KWY99_RS02185 (KWY99_02185) - 405151..405839 (-) 689 Protein_411 SprT family zinc-dependent metalloprotease -
  KWY99_RS02190 (KWY99_02190) - 405836..406579 (-) 744 WP_003687014.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  KWY99_RS02195 (KWY99_02195) gmhB 406609..407172 (-) 564 WP_003687015.1 D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase -
  KWY99_RS02200 (KWY99_02200) - 407315..408978 (+) 1664 Protein_414 LTA synthase family protein -
  KWY99_RS02205 (KWY99_02205) - 409045..410100 (-) 1056 WP_082295460.1 glycosyltransferase family 4 protein -
  KWY99_RS02210 (KWY99_02210) - 410252..411493 (-) 1242 WP_003694622.1 aromatic amino acid transporter -
  KWY99_RS02215 (KWY99_02215) ubiG 411641..412369 (+) 729 WP_003694623.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  KWY99_RS02220 (KWY99_02220) thrB 412406..413323 (+) 918 WP_003692348.1 homoserine kinase -
  KWY99_RS02225 (KWY99_02225) - 413509..414027 (-) 519 WP_003687025.1 gluconokinase, GntK/IdnK-type -
  KWY99_RS02230 (KWY99_02230) - 414047..415432 (-) 1386 WP_003694624.1 GntP family permease -
  KWY99_RS02235 (KWY99_02235) - 415823..417364 (+) 1542 WP_020997419.1 iron ABC transporter permease -
  KWY99_RS02240 (KWY99_02240) - 417393..417992 (+) 600 WP_003687033.1 carbonic anhydrase -
  KWY99_RS02245 (KWY99_02245) nadD 417989..418594 (+) 606 WP_003694625.1 nicotinate-nucleotide adenylyltransferase -
  KWY99_RS02250 (KWY99_02250) rsfS 418653..419039 (+) 387 WP_003687037.1 ribosome silencing factor -
  KWY99_RS02255 (KWY99_02255) rlmH 419092..419562 (+) 471 WP_003690383.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  KWY99_RS02260 (KWY99_02260) - 419636..419902 (+) 267 WP_002214948.1 oxidative damage protection protein -
  KWY99_RS02265 (KWY99_02265) - 420059..420742 (+) 684 WP_003687040.1 Bax inhibitor-1/YccA family protein -
  KWY99_RS02270 (KWY99_02270) coaD 420872..421387 (+) 516 WP_003692360.1 pantetheine-phosphate adenylyltransferase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16861.16 Da        Isoelectric Point: 10.3244

>NTDB_id=587064 KWY99_RS02170 WP_167549147.1 403479..403943(-) (pilE) [Neisseria gonorrhoeae strain MS11]
MSNTSKKSAVTGYCPNHGKWPKDNTSAGVASPAEIKGKYVKSVTVAKGVVTAQMNPSGVNNEIKDKKLSLWAKRENGSVK
WFCGQPVTRTDDAAKDAVTADAKDAIETKHLPSTCRDESSAGCIKTPRADFKHFQKISRCRVLPESRHMAERQR

Nucleotide


Download         Length: 465 bp        

>NTDB_id=587064 KWY99_RS02170 WP_167549147.1 403479..403943(-) (pilE) [Neisseria gonorrhoeae strain MS11]
ATTTCAAACACTTCCAAAAAATCAGCCGTTACCGGGTATTGCCCGAATCACGGCAAATGGCCGAAAGACAACACTTCTGC
CGGCGTGGCATCCCCCGCCGAAATCAAAGGCAAATATGTTAAAAGCGTTACGGTCGCAAAAGGCGTCGTTACCGCCCAAA
TGAATCCAAGCGGCGTAAACAATGAAATCAAAGACAAAAAACTCTCCCTGTGGGCCAAGCGTGAAAACGGTTCGGTAAAA
TGGTTCTGCGGACAGCCGGTTACGCGCACCGACGACGCCGCCAAAGACGCCGTTACCGCCGACGCCAAAGACGCCATCGA
AACCAAGCACCTGCCGTCAACCTGCCGCGATGAATCATCTGCCGGTTGCATAAAAACACCACGCGCCGATTTCAAACACT
TCCAAAAAATCAGCCGTTGCCGGGTATTGCCCGAATCACGGCACATGGCCGAAAGACAACGGTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

75.862

75.325

0.571
  pilE Neisseria gonorrhoeae MS11

73.729

76.623

0.565