Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   MX621_RS25290 Genome accession   NZ_CP095923
Coordinates   5362232..5362657 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain AR19438     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5357232..5367657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX621_RS25275 (MX621_25275) pilX 5357802..5358389 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  MX621_RS25280 (MX621_25280) pilY1 5358401..5361886 (+) 3486 WP_003110421.1 type 4a pilus biogenesis protein PilY1 -
  MX621_RS25285 (MX621_25285) pilY2 5361888..5362235 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  MX621_RS25290 (MX621_25290) comF 5362232..5362657 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  MX621_RS25295 (MX621_25295) ispH 5362704..5363648 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  MX621_RS25300 (MX621_25300) fkpB 5363734..5364174 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  MX621_RS25305 (MX621_25305) lspA 5364167..5364676 (-) 510 WP_003110420.1 signal peptidase II -
  MX621_RS25310 (MX621_25310) ileS 5364669..5367500 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=585689 MX621_RS25290 WP_003094721.1 5362232..5362657(+) (comF) [Pseudomonas aeruginosa strain AR19438]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=585689 MX621_RS25290 WP_003094721.1 5362232..5362657(+) (comF) [Pseudomonas aeruginosa strain AR19438]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383