Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU514_RS01685 Genome accession   NZ_CP077919
Coordinates   278497..278994 (+) Length   165 a.a.
NCBI ID   WP_029642894.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 278     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273497..283994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU514_RS01655 (KU514_01630) - 273857..275503 (+) 1647 WP_000277718.1 IS1182 family transposase -
  KU514_RS01660 (KU514_01635) comGA 275528..276502 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU514_RS01665 (KU514_01640) comGB 276474..277544 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU514_RS01670 (KU514_01645) comGC 277558..277869 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KU514_RS01675 (KU514_01650) comGD 277847..278293 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU514_RS01680 (KU514_01655) comGE 278280..278579 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU514_RS01685 (KU514_01660) comGF 278497..278994 (+) 498 WP_029642894.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU514_RS01690 (KU514_01665) - 279091..279237 (+) 147 WP_001789879.1 hypothetical protein -
  KU514_RS01695 (KU514_01670) - 279227..279751 (+) 525 WP_001015123.1 shikimate kinase -
  KU514_RS01700 (KU514_01675) gcvT 279910..281001 (+) 1092 WP_225806598.1 glycine cleavage system aminomethyltransferase GcvT -
  KU514_RS01705 (KU514_01680) gcvPA 281021..282367 (+) 1347 WP_000019689.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU514_RS01710 (KU514_01685) gcvPB 282360..283832 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19196.92 Da        Isoelectric Point: 10.2192

>NTDB_id=585074 KU514_RS01685 WP_029642894.1 278497..278994(+) (comGF) [Staphylococcus aureus strain 278]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTNVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=585074 KU514_RS01685 WP_029642894.1 278497..278994(+) (comGF) [Staphylococcus aureus strain 278]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAAATGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(70-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976