Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KU527_RS03665 Genome accession   NZ_CP077908
Coordinates   765702..766388 (-) Length   228 a.a.
NCBI ID   WP_072468915.1    Uniprot ID   A0A5F0HFN7
Organism   Staphylococcus aureus strain 322     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 760702..771388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU527_RS03645 (KU527_03630) rpsT 761616..761867 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  KU527_RS03650 (KU527_03635) holA 761912..762886 (-) 975 WP_001282573.1 DNA polymerase III subunit delta -
  KU527_RS03655 (KU527_03640) comEC 762943..765144 (-) 2202 WP_001622275.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KU527_RS03660 (KU527_03645) - 765149..765610 (-) 462 WP_000439693.1 ComE operon protein 2 -
  KU527_RS03665 (KU527_03650) comEA 765702..766388 (-) 687 WP_072468915.1 ComEA family DNA-binding protein Machinery gene
  KU527_RS03670 (KU527_03655) - 766428..767144 (-) 717 WP_000084840.1 class I SAM-dependent DNA methyltransferase -
  KU527_RS03675 (KU527_03660) rsfS 767147..767500 (-) 354 WP_001088020.1 ribosome silencing factor -
  KU527_RS03680 (KU527_03665) yqeK 767501..768085 (-) 585 WP_001017837.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KU527_RS03685 (KU527_03670) nadD 768075..768644 (-) 570 WP_000725165.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  KU527_RS03690 (KU527_03675) yhbY 768647..768937 (-) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  KU527_RS03695 (KU527_03680) aroE 768941..769747 (-) 807 WP_061104863.1 shikimate dehydrogenase -
  KU527_RS03700 (KU527_03685) yqeH 769761..770861 (-) 1101 WP_001280146.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25812.41 Da        Isoelectric Point: 9.3305

>NTDB_id=584863 KU527_RS03665 WP_072468915.1 765702..766388(-) (comEA) [Staphylococcus aureus strain 322]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQAKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGENSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=584863 KU527_RS03665 WP_072468915.1 765702..766388(-) (comEA) [Staphylococcus aureus strain 322]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGCCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACACCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGAAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HFN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961