Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU497_RS05035 Genome accession   NZ_CP077880
Coordinates   951445..951942 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 356     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 946445..956942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU497_RS04995 (KU497_04985) - 946489..947475 (+) 987 WP_000161314.1 ROK family glucokinase -
  KU497_RS05000 (KU497_04990) - 947475..947804 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU497_RS05005 (KU497_04995) - 947801..948424 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU497_RS05010 (KU497_05000) comGA 948476..949450 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  KU497_RS05015 (KU497_05005) comGB 949422..950492 (+) 1071 Protein_949 competence type IV pilus assembly protein ComGB -
  KU497_RS05020 (KU497_05010) comGC 950506..950817 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KU497_RS05025 (KU497_05015) comGD 950795..951241 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU497_RS05030 (KU497_05020) comGE 951228..951527 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU497_RS05035 (KU497_05025) comGF 951445..951942 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU497_RS05040 (KU497_05030) - 952039..952185 (+) 147 WP_001789879.1 hypothetical protein -
  KU497_RS05045 (KU497_05035) - 952175..952699 (+) 525 WP_001015117.1 shikimate kinase -
  KU497_RS05050 (KU497_05040) gcvT 952858..953949 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KU497_RS05055 (KU497_05045) gcvPA 953969..955315 (+) 1347 WP_048665334.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU497_RS05060 (KU497_05050) gcvPB 955308..956780 (+) 1473 WP_050956458.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=584489 KU497_RS05035 WP_001789864.1 951445..951942(+) (comGF) [Staphylococcus aureus strain 356]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=584489 KU497_RS05035 WP_001789864.1 951445..951942(+) (comGF) [Staphylococcus aureus strain 356]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964