Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KU528_RS01025 Genome accession   NZ_CP077865
Coordinates   228698..229384 (-) Length   228 a.a.
NCBI ID   WP_078055782.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 370     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 223698..234384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU528_RS01005 (KU528_00995) rpsT 224612..224863 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  KU528_RS01010 (KU528_01000) holA 224908..225882 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  KU528_RS01015 (KU528_01005) comEC 225939..228140 (-) 2202 WP_225805745.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KU528_RS01020 (KU528_01010) - 228145..228606 (-) 462 WP_000439693.1 ComE operon protein 2 -
  KU528_RS01025 (KU528_01015) comEA 228698..229384 (-) 687 WP_078055782.1 ComEA family DNA-binding protein Machinery gene
  KU528_RS01030 (KU528_01020) - 229424..230140 (-) 717 WP_000942456.1 class I SAM-dependent DNA methyltransferase -
  KU528_RS01035 (KU528_01025) rsfS 230143..230496 (-) 354 WP_001088020.1 ribosome silencing factor -
  KU528_RS01040 (KU528_01030) yqeK 230497..231081 (-) 585 WP_001017838.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KU528_RS01045 (KU528_01035) nadD 231071..231640 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  KU528_RS01050 (KU528_01040) yhbY 231643..231933 (-) 291 WP_000955233.1 ribosome assembly RNA-binding protein YhbY -
  KU528_RS01055 (KU528_01045) aroE 231937..232743 (-) 807 WP_000666767.1 shikimate dehydrogenase -
  KU528_RS01060 (KU528_01050) yqeH 232757..233857 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25761.45 Da        Isoelectric Point: 9.6753

>NTDB_id=584182 KU528_RS01025 WP_078055782.1 228698..229384(-) (comEA) [Staphylococcus aureus strain 370]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=584182 KU528_RS01025 WP_078055782.1 228698..229384(-) (comEA) [Staphylococcus aureus strain 370]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCTAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTGCTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
GAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.678

99.561

0.982
  comEA Staphylococcus aureus N315

98.678

99.561

0.982