Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU521_RS02375 Genome accession   NZ_CP077860
Coordinates   516572..517069 (-) Length   165 a.a.
NCBI ID   WP_078098371.1    Uniprot ID   -
Organism   Staphylococcus aureus strain AC4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511572..522069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU521_RS02350 (KU521_02340) gcvPB 511734..513206 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  KU521_RS02355 (KU521_02345) gcvPA 513199..514545 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU521_RS02360 (KU521_02350) gcvT 514565..515656 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KU521_RS02365 (KU521_02355) - 515815..516339 (-) 525 WP_001015120.1 shikimate kinase -
  KU521_RS02370 (KU521_02360) - 516329..516475 (-) 147 WP_001792109.1 hypothetical protein -
  KU521_RS02375 (KU521_02365) comGF 516572..517069 (-) 498 WP_078098371.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU521_RS02380 (KU521_02370) comGE 516987..517286 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  KU521_RS02385 (KU521_02375) comGD 517273..517719 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU521_RS02390 (KU521_02380) comGC 517697..518008 (-) 312 WP_031763468.1 competence type IV pilus major pilin ComGC Machinery gene
  KU521_RS02395 (KU521_02385) comGB 518022..519092 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU521_RS02400 (KU521_02390) comGA 519064..520038 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU521_RS02405 (KU521_02395) - 520090..520713 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU521_RS02410 (KU521_02400) - 520710..521039 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU521_RS02415 (KU521_02405) - 521039..522025 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19155.01 Da        Isoelectric Point: 10.4428

>NTDB_id=584051 KU521_RS02375 WP_078098371.1 516572..517069(-) (comGF) [Staphylococcus aureus strain AC4]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEKMIEHKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=584051 KU521_RS02375 WP_078098371.1 516572..517069(-) (comGF) [Staphylococcus aureus strain AC4]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAAAAAT
GATCGAACACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

95.152

100

0.952