Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   B1NLA3E_RS04940 Genome accession   NC_021171
Coordinates   1007609..1011400 (+) Length   1263 a.a.
NCBI ID   WP_015592741.1    Uniprot ID   N0APL6
Organism   Bacillus sp. 1NLA3E     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1002609..1016400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1NLA3E_RS04920 (B1NLA3E_04500) - 1002732..1002998 (+) 267 WP_041580316.1 PqqD family protein -
  B1NLA3E_RS23205 (B1NLA3E_04505) - 1002988..1003485 (+) 498 WP_015592738.1 S24/S26 family peptidase -
  B1NLA3E_RS04930 (B1NLA3E_04510) - 1003617..1003868 (-) 252 WP_041580317.1 TfoX/Sxy family protein -
  B1NLA3E_RS04935 (B1NLA3E_04515) addB 1004134..1007640 (+) 3507 WP_015592740.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  B1NLA3E_RS04940 (B1NLA3E_04520) addA 1007609..1011400 (+) 3792 WP_015592741.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  B1NLA3E_RS04945 (B1NLA3E_04525) - 1011494..1011712 (-) 219 WP_015592742.1 spore germination protein -
  B1NLA3E_RS04950 (B1NLA3E_04530) - 1011766..1012179 (-) 414 WP_015592743.1 spore germination protein GerPE -
  B1NLA3E_RS04955 (B1NLA3E_04535) - 1012181..1012363 (-) 183 WP_015592744.1 germination protein GerPD -
  B1NLA3E_RS04960 (B1NLA3E_04540) - 1012378..1012977 (-) 600 WP_015592745.1 spore germination protein GerPC -
  B1NLA3E_RS04965 (B1NLA3E_04545) - 1013025..1013243 (-) 219 WP_015592746.1 spore germination protein GerPB -
  B1NLA3E_RS04970 (B1NLA3E_04550) - 1013252..1013473 (-) 222 WP_015592747.1 spore germination protein -
  B1NLA3E_RS04975 (B1NLA3E_04555) - 1013663..1015261 (+) 1599 WP_015592748.1 long-chain-fatty-acid--CoA ligase -

Sequence

Protein


Download         Length: 1263 a.a.        Molecular weight: 144972.82 Da        Isoelectric Point: 5.1049

>NTDB_id=58386 B1NLA3E_RS04940 WP_015592741.1 1007609..1011400(+) (addA) [Bacillus sp. 1NLA3E]
MVQSIIPPKPDEVNWTDDQWKAIYAKNQDILVAAAAGSGKTAVLVERMIQRILSAENPVNVDELLVVTFTNASAAEMRHR
IGEALEKAIQADPKSVNLRKQLTLLNRASISTLHSFCLDVIRKYYYLIDIDPSFRIADETEAQLLRDEVIDELFEEEYAN
ADNEAFFALVDSFTNDRSDQALQTIIHDVYDFAMANSSPTNYLKSMITMYEVDETSQIENLPFIQVLLEDIELQLQGSKQ
LLQQGLNLTMLPAGPAPRAENFTADIQYVDTLLAAKSKSWDALYSLIQTVPFSRAKTCKGDQYDPILMENAQNLRKRAKK
IITDIQAELFSRRPQSFLNDMQEVRHYIETIVKLVHSFSTRFSAVKKEKGLVDFSDLEHYCLDLLTEKFAEDGSRMPSEA
ALFYQNRFKEVLVDEYQDTNMVQEAILQLVTKGKEESGNLFMVGDVKQSIYRFRLAEPNLFLGKYQRFSMDGTDGGFRID
LAQNFRSRKEILDGTNYLFKQLMGEKVGEIKYDDAAELKLGANYPVEGDFPIELILIDQADKVDKSESLEPPEEGSSDSG
FDLNDLEQSELEARMIAIKIKTMFAERKQVYNAKTNSSKPVMFRDIVILLRSMTWAPHMLEELKRQGIPVYAKLSSGYFE
ATEVAIMMSLLKIIDNPYQDIPLAAVLRSPIVGLTEAELAEIRINQKSGTFYEAFALFCQQKPSENQEELFEKIRPFFER
LTDWRTRARQGALSQFIWQLLRETRFYEFVSGMPGGKQRQANLRALFDRARVYEATSFRGLFRFLRFIERMRDRGNDLGA
AHALGEQEDVVRIMTIHSSKGLEFPVVFMAGLNRKFNMMDIRKPYMLDKEFGFAAKYVDIEKRISYPSLPQLVFKRKKKM
EMLAEEMRVLYVALTRAKEKLYLLASVKDLQKSIDKWMQLKEHQDWLLPEYDRATASAFLDWVGPALIRHHDCEALTTSA
DQESPPALVSSEICEHASHWKIVNIAADEVAAIEEKSNEEEQTVMDFVQKGIRTPISTDAYQIVKEQLSWKYEHIEASKH
LSKQSVSEIKRQQEIFNHGSGTELQHKFSKPLFKRPRFMQDKALTPAERGTAMHMVMQHVDFTKAINDFAIHALLDRMVE
NELLSIEQKEVIDADRIVAFFQTELGNRLANASRIKREVPFSLSIPASRVYADWNGKDDEQVLVQGIIDCLFEDEQGVVL
LDYKTDAITDRFKGGFEEAMPVLAERYRVQINLYAKAVEQILKKPVEEKFLFFFDGGHLLKID

Nucleotide


Download         Length: 3792 bp        

>NTDB_id=58386 B1NLA3E_RS04940 WP_015592741.1 1007609..1011400(+) (addA) [Bacillus sp. 1NLA3E]
TTGGTTCAATCGATCATACCTCCAAAACCTGATGAAGTGAATTGGACAGATGACCAGTGGAAAGCAATTTATGCAAAAAA
CCAAGATATCCTCGTTGCCGCAGCAGCTGGGTCGGGAAAAACGGCGGTTTTAGTAGAACGAATGATTCAGAGAATTCTTT
CGGCAGAGAATCCGGTAAATGTGGATGAACTTTTGGTCGTGACGTTTACCAATGCCTCGGCTGCGGAGATGAGGCATCGA
ATTGGGGAGGCCTTGGAGAAAGCAATTCAGGCTGATCCAAAATCAGTTAATCTTAGAAAACAATTAACGTTATTAAATCG
GGCATCCATTTCAACCTTGCATTCCTTCTGTTTAGATGTCATTCGAAAATACTATTATTTAATTGATATAGATCCCAGTT
TTCGTATTGCCGATGAAACCGAGGCGCAACTGCTGCGTGATGAAGTGATCGATGAGTTATTTGAGGAAGAGTATGCCAAT
GCCGACAATGAGGCTTTTTTCGCTTTAGTGGATTCATTTACAAACGATCGCAGTGATCAAGCACTACAAACTATCATTCA
CGATGTATATGATTTTGCCATGGCCAATTCATCACCTACAAACTACTTGAAATCGATGATAACGATGTATGAGGTTGATG
AGACGTCTCAAATCGAGAACCTCCCGTTTATTCAGGTGTTGCTTGAAGATATCGAATTACAGCTCCAAGGTTCGAAGCAA
TTGCTCCAGCAAGGGCTGAATCTCACCATGCTACCAGCGGGTCCGGCGCCAAGGGCAGAAAACTTTACCGCTGATATCCA
ATATGTTGATACTTTGTTAGCTGCAAAAAGTAAATCATGGGATGCACTATATTCATTGATTCAAACGGTCCCTTTTTCAA
GAGCAAAAACTTGTAAAGGGGATCAATATGATCCGATTTTAATGGAAAATGCCCAAAACTTACGTAAAAGGGCTAAAAAA
ATTATTACCGATATTCAAGCAGAACTTTTTTCCAGGAGGCCTCAAAGCTTTCTGAACGATATGCAGGAAGTAAGGCATTA
TATCGAAACGATTGTAAAGCTTGTTCATTCTTTTTCGACCCGTTTTTCGGCGGTGAAAAAGGAAAAGGGACTGGTTGATT
TTTCTGATTTAGAGCATTACTGTTTGGATTTACTAACCGAAAAATTTGCGGAAGATGGCTCAAGAATGCCATCGGAGGCT
GCTTTATTTTACCAAAATCGCTTTAAAGAGGTTTTGGTTGATGAATACCAAGATACGAATATGGTTCAAGAAGCGATACT
ACAGCTCGTGACAAAAGGCAAAGAGGAATCAGGGAATTTATTTATGGTTGGTGACGTGAAGCAGTCGATATACCGTTTCC
GCCTAGCTGAACCCAATTTATTTCTTGGAAAGTACCAGCGGTTTTCTATGGATGGAACGGATGGTGGTTTTCGAATCGAT
CTAGCCCAGAACTTCAGAAGCCGTAAGGAAATTCTCGACGGCACTAATTATTTATTTAAACAGCTAATGGGTGAAAAGGT
TGGAGAAATCAAATATGATGACGCCGCTGAATTGAAGCTTGGTGCAAACTATCCAGTTGAGGGAGATTTTCCTATTGAAC
TCATCTTAATCGATCAAGCGGACAAGGTGGACAAGAGCGAAAGTTTAGAACCACCAGAAGAAGGTAGTAGCGATTCGGGA
TTTGATCTTAATGATCTTGAACAATCGGAACTCGAAGCTAGGATGATCGCTATAAAAATAAAAACAATGTTCGCGGAACG
AAAGCAAGTTTATAATGCAAAAACCAATTCTTCAAAACCAGTGATGTTCAGGGATATCGTTATTTTGCTACGCTCGATGA
CATGGGCTCCACATATGCTTGAGGAATTAAAACGGCAAGGAATTCCGGTCTATGCTAAATTATCATCGGGATATTTTGAG
GCGACAGAAGTGGCGATTATGATGTCTTTATTAAAAATCATTGACAATCCATATCAAGACATCCCGCTAGCAGCTGTATT
ACGGTCGCCAATCGTCGGCTTAACTGAGGCTGAGCTAGCTGAAATTCGAATCAATCAGAAAAGCGGGACATTTTATGAAG
CCTTCGCCTTATTTTGTCAGCAGAAGCCTTCGGAAAATCAGGAGGAGCTGTTTGAAAAAATTCGGCCATTTTTTGAGCGA
TTGACAGACTGGAGAACGAGGGCACGGCAAGGAGCCTTGTCCCAGTTCATTTGGCAGTTATTACGTGAAACAAGATTTTA
TGAATTCGTTAGCGGCATGCCAGGAGGCAAACAACGGCAAGCCAACCTGCGGGCCTTGTTTGACCGAGCCAGAGTATATG
AAGCAACGTCATTTAGAGGACTGTTTCGGTTTTTACGTTTCATCGAGCGGATGAGGGATCGTGGTAATGACCTTGGTGCT
GCCCATGCTCTTGGTGAACAAGAGGACGTTGTCCGCATCATGACGATTCACAGCAGTAAAGGGTTGGAATTCCCAGTGGT
TTTCATGGCTGGGCTCAATCGCAAATTTAATATGATGGATATAAGAAAACCGTATATGCTAGATAAGGAATTTGGATTTG
CCGCAAAATACGTCGATATTGAAAAACGAATTTCCTATCCTTCATTACCACAGTTGGTATTTAAACGAAAAAAGAAAATG
GAAATGTTAGCTGAGGAAATGCGTGTCCTTTATGTTGCGTTGACACGAGCGAAGGAAAAGCTTTATTTGTTAGCGTCTGT
AAAGGATTTACAGAAATCGATTGACAAGTGGATGCAACTGAAAGAACACCAAGACTGGCTTTTACCTGAGTACGATCGGG
CAACAGCCTCAGCATTCTTAGATTGGGTCGGCCCAGCACTTATTCGTCATCATGATTGTGAAGCTTTAACCACCTCAGCT
GACCAAGAAAGCCCGCCTGCATTAGTCTCAAGTGAGATTTGTGAGCACGCTTCACATTGGAAGATTGTTAATATAGCAGC
AGATGAGGTAGCGGCTATTGAAGAGAAAAGCAATGAGGAAGAGCAAACTGTGATGGACTTTGTTCAAAAAGGGATCCGAA
CGCCGATCTCAACTGATGCTTACCAAATTGTGAAGGAACAGCTTTCCTGGAAATATGAGCATATCGAAGCATCCAAACAT
CTTTCTAAACAATCAGTATCGGAAATAAAGCGGCAACAGGAGATTTTTAATCATGGTAGTGGCACTGAGTTGCAGCATAA
ATTTTCAAAACCGCTGTTTAAGAGGCCTAGATTCATGCAGGATAAAGCCTTGACGCCTGCCGAACGGGGAACGGCGATGC
ATATGGTGATGCAGCATGTAGATTTTACCAAAGCGATTAATGATTTTGCAATTCATGCATTATTGGATCGAATGGTTGAA
AATGAACTTTTATCAATTGAGCAAAAAGAAGTGATTGATGCCGACCGTATTGTTGCTTTTTTCCAAACTGAGTTAGGTAA
TCGTCTTGCAAATGCTAGTAGGATTAAACGCGAAGTTCCGTTCAGCTTGTCCATTCCTGCATCTCGGGTTTATGCCGATT
GGAATGGGAAAGATGATGAACAGGTGCTCGTCCAAGGGATTATCGACTGTTTATTTGAGGATGAGCAAGGCGTTGTGTTG
CTTGATTATAAAACAGATGCGATTACTGACCGATTCAAGGGTGGCTTTGAAGAAGCGATGCCAGTGTTAGCAGAGCGGTA
CAGAGTTCAAATCAACTTATACGCCAAAGCGGTTGAACAAATATTAAAGAAACCTGTCGAGGAGAAGTTCTTATTTTTCT
TTGACGGCGGCCATTTGTTGAAGATCGATTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(479-905)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1077-1245)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N0APL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.302

99.525

0.53

Multiple sequence alignment