Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   B1NLA3E_RS04935 Genome accession   NC_021171
Coordinates   1004134..1007640 (+) Length   1168 a.a.
NCBI ID   WP_015592740.1    Uniprot ID   N0ARJ4
Organism   Bacillus sp. 1NLA3E     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 999134..1012640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1NLA3E_RS04910 (B1NLA3E_04490) - 999900..1001696 (+) 1797 WP_015592735.1 ABC transporter ATP-binding protein -
  B1NLA3E_RS04915 (B1NLA3E_04495) - 1001971..1002450 (+) 480 WP_187292152.1 VanZ family protein -
  B1NLA3E_RS04920 (B1NLA3E_04500) - 1002732..1002998 (+) 267 WP_041580316.1 PqqD family protein -
  B1NLA3E_RS23205 (B1NLA3E_04505) - 1002988..1003485 (+) 498 WP_015592738.1 S24/S26 family peptidase -
  B1NLA3E_RS04930 (B1NLA3E_04510) - 1003617..1003868 (-) 252 WP_041580317.1 TfoX/Sxy family protein -
  B1NLA3E_RS04935 (B1NLA3E_04515) addB 1004134..1007640 (+) 3507 WP_015592740.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  B1NLA3E_RS04940 (B1NLA3E_04520) addA 1007609..1011400 (+) 3792 WP_015592741.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  B1NLA3E_RS04945 (B1NLA3E_04525) - 1011494..1011712 (-) 219 WP_015592742.1 spore germination protein -
  B1NLA3E_RS04950 (B1NLA3E_04530) - 1011766..1012179 (-) 414 WP_015592743.1 spore germination protein GerPE -
  B1NLA3E_RS04955 (B1NLA3E_04535) - 1012181..1012363 (-) 183 WP_015592744.1 germination protein GerPD -

Sequence

Protein


Download         Length: 1168 a.a.        Molecular weight: 134712.36 Da        Isoelectric Point: 6.1655

>NTDB_id=58385 B1NLA3E_RS04935 WP_015592740.1 1004134..1007640(+) (addB) [Bacillus sp. 1NLA3E]
MSVRFIIGRSGSGKTQKCLNEMRERLTVDPGGPPIIYLVPEQMTFLSEHRLSTSPQLGGMIRAQVYSFTRLAWRVLQETG
GFNRYHLNSTGISMLIRKIIEEKKDELTLFKRAADKNGFIEQMEQLLTEFKRYCIQPEELAEKSGQMAQQKSLHDKLGDL
ELIYQHFEEALQDKYIDSEDYFRLLAEKAASSKFLQEAEIYIDGFYSFTPIEYMVIEQLMKHCKRVTISLTLDQPFTKSL
PEELHLFRMTGDTCQTLHDMVRAEGIPYEDPILLTELLRWKNHSFFHLEKYFDLLPVQEYRGETAIHIAQAVNRRAEMEG
VARKITELVRKHGYRYREIAILSRNSHEYHDVLEVIFADCGIPFYIDQKTSMQHHPVIELLRSSLEILNGNWRYEPIFRA
VKTELLFPFDMNLLKLREKMDKLENYVLAYGIQGDKWTRKQQWTYRRFRGLELESLPQTDSEKKIEDELNELRGMITSPL
LRLSRRLKKAKTGMELSEALYLFLEELEIPRKLERWKQDEEAKGNLVKAREHDQVWDAVIELIDQFVEMLGNEKIAAKQF
AVIIESGIESLRFSLVPPAMDQVVVADLEKSRLEDIKAAFIIGLNEGVLPAKLTDEGIFGDEDREALLANGLKIAPSSRT
RLLDENFLAYKAFTLPSEVLFLSYPVANEEGKALMPSPYIKRMHELFPQAIGHLFVADPAELSEMEQLAYVSNENTTLTY
LTAQLQLKQQHYPIYDFWWDVYNYYMENDQWRTTATKVLSSLFYDNKTSKLPEQVSHELYGDLIQASVSRMEMFHSCPFS
HFAHHGLKLRDRQIFRLDAPDIGELFHAALKQISDTVNQQNLSWSQITRTQCEAMAGQAVEMLAPRLQNEILLSSNRNVY
LKRKLEKIISRATYVLSEHAKVSGFSPVGLELGFGPKGDLPPISFSLKNGTKMELVGRIDRVDKANDGENVFLRVIDYKS
SERELNISEVYYGLSLQMLTYLDIIITHSQQLVGTDASPAGVLYFHVHNPIIQTTKMLSLDDIEREMFKRFKMNGLILGE
QNVIHLMDQTLESGESNMIPVGINKDGTLRKQSKVVNRGEFEQLTKYVRDTYVKSGNQMLDGAVSISPYKTTNKSACTFC
SFKSICHFDPSIKNNEYRNLPNMSKEEILESIQKEDSSLGSIDHTSKT

Nucleotide


Download         Length: 3507 bp        

>NTDB_id=58385 B1NLA3E_RS04935 WP_015592740.1 1004134..1007640(+) (addB) [Bacillus sp. 1NLA3E]
ATGTCTGTTCGATTTATTATTGGGCGGTCAGGTAGCGGTAAGACTCAAAAATGCCTAAACGAAATGAGGGAGAGGCTGAC
GGTTGATCCCGGTGGTCCTCCAATTATTTATTTAGTGCCAGAGCAAATGACTTTTTTATCCGAGCATCGGCTGTCAACTT
CACCTCAGCTTGGCGGAATGATTCGCGCCCAAGTATATAGTTTTACCCGTTTAGCATGGCGAGTTCTCCAAGAGACGGGC
GGGTTTAATCGTTACCATTTGAATAGCACCGGAATCAGCATGCTGATTCGAAAGATTATTGAAGAAAAGAAGGACGAACT
TACACTGTTTAAACGAGCTGCCGATAAAAATGGTTTTATCGAGCAAATGGAGCAATTGTTAACAGAATTTAAGCGTTATT
GCATTCAACCGGAAGAGCTTGCCGAAAAATCCGGACAAATGGCCCAGCAAAAATCACTTCATGACAAGCTAGGCGACCTT
GAATTGATTTATCAACACTTTGAAGAGGCTTTACAGGATAAATATATTGATTCCGAAGATTATTTTCGTCTCCTAGCAGA
GAAGGCAGCTTCATCAAAATTTTTGCAAGAGGCGGAAATCTATATAGATGGCTTTTACAGCTTTACACCGATTGAATATA
TGGTGATTGAGCAATTGATGAAGCATTGCAAACGGGTGACCATTTCGTTAACGTTGGATCAACCCTTTACGAAATCCCTT
CCTGAGGAGCTTCATCTTTTTCGCATGACGGGTGACACGTGTCAAACTCTGCACGACATGGTTAGAGCCGAGGGAATCCC
TTATGAAGACCCGATATTGTTAACAGAGCTATTACGCTGGAAAAATCACTCTTTTTTCCATCTTGAAAAATATTTTGATT
TGTTGCCTGTACAGGAATACAGGGGTGAAACAGCGATTCACATCGCACAAGCGGTGAACAGAAGAGCAGAAATGGAAGGA
GTCGCTCGAAAAATAACCGAACTCGTTCGTAAACATGGCTATCGTTACCGCGAAATTGCAATATTATCGAGAAACAGTCA
TGAATATCATGATGTTTTGGAAGTCATTTTTGCTGACTGTGGAATTCCATTTTACATCGATCAAAAGACATCGATGCAGC
ATCATCCTGTAATCGAACTTCTCCGCTCAAGTCTGGAAATCCTCAATGGGAATTGGCGCTATGAGCCAATTTTTCGTGCC
GTTAAAACCGAACTCCTTTTCCCATTTGATATGAATCTATTAAAACTTCGTGAAAAAATGGACAAATTGGAGAACTATGT
ATTAGCCTATGGGATTCAAGGCGATAAATGGACGCGAAAGCAGCAATGGACATACCGAAGATTTAGAGGTTTAGAGCTTG
AAAGTCTACCGCAAACGGACTCCGAGAAGAAAATCGAGGACGAGTTAAATGAGTTAAGGGGAATGATTACGAGCCCATTG
TTGCGTCTCTCCCGAAGATTGAAAAAGGCTAAAACCGGAATGGAGTTAAGCGAGGCATTATATTTATTTTTAGAAGAATT
AGAGATTCCGCGCAAGCTTGAACGCTGGAAACAGGATGAAGAGGCAAAAGGGAATCTGGTTAAGGCGCGAGAGCATGATC
AGGTTTGGGATGCGGTAATCGAGTTAATCGATCAATTTGTCGAAATGCTAGGTAATGAAAAAATAGCGGCTAAACAATTT
GCAGTGATTATCGAATCAGGGATTGAATCGCTACGCTTTTCCCTTGTACCACCGGCAATGGATCAAGTAGTGGTAGCTGA
TCTGGAGAAGTCAAGGCTTGAAGACATTAAGGCCGCCTTTATCATTGGCTTAAATGAAGGTGTTTTGCCGGCAAAGTTAA
CTGATGAGGGAATTTTTGGAGATGAAGATCGAGAAGCTTTGTTAGCAAATGGTTTGAAAATTGCTCCATCGAGCCGGACC
CGCTTACTCGATGAAAATTTCCTTGCTTATAAGGCGTTTACCTTGCCATCGGAAGTGCTGTTCTTAAGTTATCCGGTCGC
TAATGAAGAAGGAAAAGCGTTAATGCCCTCTCCTTATATAAAAAGAATGCATGAATTGTTTCCACAGGCAATTGGACATT
TATTTGTCGCAGATCCCGCTGAATTGTCCGAAATGGAGCAACTTGCGTATGTAAGCAATGAAAATACAACGCTTACCTAT
TTGACGGCGCAGTTGCAATTAAAGCAGCAACATTATCCGATTTACGATTTTTGGTGGGACGTATATAACTATTATATGGA
AAATGATCAATGGCGAACAACGGCAACCAAGGTCTTATCGAGTTTATTTTATGATAATAAGACAAGCAAGCTTCCAGAGC
AAGTCAGCCATGAACTCTACGGTGACCTAATTCAAGCGAGCGTGTCGCGGATGGAAATGTTTCATAGCTGTCCGTTTTCC
CATTTTGCACACCATGGGTTAAAGTTGCGGGATCGCCAAATATTCCGTTTGGATGCCCCTGATATCGGTGAACTATTTCA
TGCAGCTTTAAAGCAAATTTCCGATACTGTTAATCAACAAAATTTATCGTGGTCGCAAATCACGAGGACCCAGTGTGAGG
CGATGGCTGGGCAAGCAGTGGAAATGTTGGCACCTAGGCTACAAAATGAAATTCTGCTTAGTTCAAATCGAAATGTGTAT
TTGAAACGAAAGCTTGAAAAAATTATCAGTAGAGCGACCTATGTCTTGAGCGAGCATGCGAAAGTCAGTGGATTTTCTCC
AGTTGGTCTTGAGCTTGGTTTCGGACCAAAGGGTGACCTTCCACCGATCAGTTTTTCATTGAAGAATGGAACGAAGATGG
AGCTAGTCGGACGGATTGACCGTGTAGATAAAGCTAATGACGGTGAAAACGTCTTTTTAAGAGTAATCGATTATAAATCA
AGTGAACGGGAATTGAATATAAGTGAAGTGTATTATGGGTTATCGCTGCAAATGTTAACGTACCTAGATATCATTATTAC
TCATTCCCAGCAATTAGTTGGTACGGATGCATCACCAGCGGGAGTGTTATATTTTCATGTTCACAATCCGATTATTCAAA
CGACGAAAATGCTGTCTTTAGATGATATCGAACGAGAAATGTTTAAACGATTTAAAATGAATGGACTCATACTTGGTGAA
CAAAATGTCATTCATTTAATGGATCAAACATTGGAATCAGGAGAGTCAAACATGATTCCGGTGGGGATAAATAAGGACGG
GACCTTGAGGAAGCAATCAAAGGTAGTAAATCGTGGCGAATTCGAACAGTTAACAAAGTATGTGAGAGATACTTATGTGA
AATCAGGTAACCAAATGCTTGATGGGGCAGTGTCTATTTCTCCGTATAAAACAACTAATAAATCAGCCTGCACTTTTTGT
TCATTCAAATCGATTTGTCACTTTGATCCTTCGATAAAAAATAACGAGTATCGGAATTTGCCAAATATGTCTAAAGAAGA
GATACTCGAATCCATTCAAAAGGAGGATTCATCACTTGGTTCAATCGATCATACCTCCAAAACCTGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(786-1127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N0ARJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.987

99.743

0.509

Multiple sequence alignment