Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   KTE14_RS00290 Genome accession   NZ_CP077738
Coordinates   72342..73691 (-) Length   449 a.a.
NCBI ID   WP_000815652.1    Uniprot ID   Q8VUV4
Organism   Staphylococcus aureus strain UMCG578     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 41427..79298 72342..73691 within 0


Gene organization within MGE regions


Location: 41427..79298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KTE14_RS00165 (KTE14_00165) ccrC 41427..43106 (+) 1680 WP_031863960.1 cassette chromosome recombinase CcrC -
  KTE14_RS00170 (KTE14_00170) - 43195..43533 (+) 339 WP_031863961.1 SAUGI family uracil-DNA glycosylase inhibitor -
  KTE14_RS00175 (KTE14_00175) - 43629..43940 (+) 312 WP_031863962.1 DUF960 domain-containing protein -
  KTE14_RS00180 (KTE14_00180) - 43956..44465 (+) 510 WP_031863963.1 DUF1643 domain-containing protein -
  KTE14_RS00185 (KTE14_00185) - 44480..44701 (+) 222 WP_031863964.1 hypothetical protein -
  KTE14_RS00190 (KTE14_00190) - 44775..47894 (-) 3120 WP_031863965.1 type I restriction endonuclease subunit R -
  KTE14_RS00195 (KTE14_00195) - 47878..49143 (-) 1266 WP_049948422.1 restriction endonuclease subunit S -
  KTE14_RS00200 (KTE14_00200) - 49133..50647 (-) 1515 WP_031863967.1 type I restriction-modification system subunit M -
  KTE14_RS00205 (KTE14_00205) - 51449..56254 (+) 4806 WP_223596992.1 putative Ig domain-containing protein -
  KTE14_RS00210 (KTE14_00210) - 56438..57702 (-) 1265 Protein_39 ISL3 family transposase -
  KTE14_RS00215 (KTE14_00215) - 57885..58244 (+) 360 WP_223596994.1 antibiotic biosynthesis monooxygenase family protein -
  KTE14_RS00220 (KTE14_00220) - 58696..59951 (+) 1256 Protein_41 MrcB family domain-containing protein -
  KTE14_RS00225 (KTE14_00225) - 60366..60605 (+) 240 WP_001282728.1 hypothetical protein -
  KTE14_RS00230 (KTE14_00230) - 60637..61311 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  KTE14_RS00235 (KTE14_00235) - 61347..61736 (+) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  KTE14_RS00240 (KTE14_00240) - 62613..63356 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  KTE14_RS00245 (KTE14_00245) - 63453..63881 (+) 429 WP_000872606.1 MaoC family dehydratase -
  KTE14_RS00250 (KTE14_00250) mecA 63927..65936 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  KTE14_RS00255 (KTE14_00255) - 66033..67019 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  KTE14_RS00260 (KTE14_00260) - 67009..67251 (+) 243 Protein_49 type I restriction enzyme endonuclease domain-containing protein -
  KTE14_RS00265 (KTE14_00265) - 67242..68852 (-) 1611 Protein_50 IS1182 family transposase -
  KTE14_RS00270 (KTE14_00270) - 68901..69407 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  KTE14_RS00275 (KTE14_00275) - 69422..69733 (-) 312 WP_000700851.1 DUF960 family protein -
  KTE14_RS00280 (KTE14_00280) - 69820..70170 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  KTE14_RS00285 (KTE14_00285) ccrB 70692..72320 (-) 1629 WP_001186604.1 cassette chromosome recombinase CcrB Machinery gene
  KTE14_RS00290 (KTE14_00290) ccrA 72342..73691 (-) 1350 WP_000815652.1 cassette chromosome recombinase CcrA Machinery gene
  KTE14_RS00295 (KTE14_00295) cch1 73925..75712 (-) 1788 WP_000122621.1 cassette chromosome replicative helicase -
  KTE14_RS00300 (KTE14_00300) ssb 75712..76002 (-) 291 WP_001036437.1 cassette chromosome ssDNA-binding protein -
  KTE14_RS00305 (KTE14_00305) - 76141..77190 (+) 1050 WP_000613248.1 hypothetical protein -
  KTE14_RS00310 (KTE14_00310) - 77808..79298 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52616.30 Da        Isoelectric Point: 10.0062

>NTDB_id=582548 KTE14_RS00290 WP_000815652.1 72342..73691(-) (ccrA) [Staphylococcus aureus strain UMCG578]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHILTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQAMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=582548 KTE14_RS00290 WP_000815652.1 72342..73691(-) (ccrA) [Staphylococcus aureus strain UMCG578]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACATATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATAAATGGGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAGCCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

74.833

100

0.748