Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   MUK77_RS23655 Genome accession   NZ_CP095086
Coordinates   4944893..4947091 (+) Length   732 a.a.
NCBI ID   WP_001468126.1    Uniprot ID   -
Organism   Escherichia coli isolate EC5654     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4939893..4952091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUK77_RS23635 (MUK77_23635) metB 4941995..4943155 (-) 1161 WP_000197201.1 cystathionine gamma-synthase -
  MUK77_RS23640 (MUK77_23640) metJ 4943432..4943749 (+) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  MUK77_RS23645 (MUK77_23645) yiiX 4943809..4944417 (+) 609 WP_000702302.1 YiiX family permuted papain-like enzyme -
  MUK77_RS23650 (MUK77_23650) rpmE 4944478..4944690 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  MUK77_RS23655 (MUK77_23655) priA 4944893..4947091 (+) 2199 WP_001468126.1 primosomal protein N' Machinery gene
  MUK77_RS23660 (MUK77_23660) cytR 4947247..4948272 (+) 1026 WP_000644911.1 DNA-binding transcriptional regulator CytR Regulator
  MUK77_RS23665 (MUK77_23665) ftsN 4948364..4949323 (+) 960 WP_000068834.1 cell division protein FtsN -
  MUK77_RS23670 (MUK77_23670) hslV 4949416..4949946 (+) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  MUK77_RS23675 (MUK77_23675) hslU 4949956..4951287 (+) 1332 WP_001293344.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81758.06 Da        Isoelectric Point: 9.1153

>NTDB_id=582052 MUK77_RS23655 WP_001468126.1 4944893..4947091(+) (priA) [Escherichia coli isolate EC5654]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGYRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSSLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPVLLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMHLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=582052 MUK77_RS23655 WP_001468126.1 4944893..4947091(+) (priA) [Escherichia coli isolate EC5654]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTATCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGCG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTGTTTACTCACTCCGTCTGGCGATTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTACGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GCTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCGACCAAGTTGCCACGCTTGAATTT
AATGATGCCGCGTTACAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAAGTTTATCTCAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCAGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACACCCGCGCTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCGGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGTCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGTGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTGCCGGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGGTGTCCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAACAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCATTACTGGACGTAGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTCGCCCAGCTTTACACCCAAGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGTGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACATTGCTGTATAAAGGCT
ATGACGCCTTTGCAGAACAGGCGCTTGCCGAGCGGCGAATGATGCATTTGCCGCCGTGGACCAGTCATGTGATTGTGCGC
GCCGAAGATCATAACAATCAACATGCACCACTGTTCCTGCAACAGTTGCGAAATCTCATCCTCTCCAGCCCACTGGCGGA
TGACAAGCTCTGGGTACTCGGCCCGGTTCCGGCACTGGCCCCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTCGATCCCATCGAGGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

DEAD/DEAH box helicase (DEAD)

(203-362)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451