Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   LEUCM_RS10015 Genome accession   NZ_AP017929
Coordinates   1529280..1530038 (-) Length   252 a.a.
NCBI ID   WP_035146927.1    Uniprot ID   -
Organism   Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1524280..1535038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEUCM_RS07630 (LACBS_01511) addA 1526305..1529331 (-) 3027 WP_258421054.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  LEUCM_RS10015 (LACBS_01512) addA 1529280..1530038 (-) 759 WP_035146927.1 UvrD-helicase domain-containing protein Machinery gene
  LEUCM_RS07635 (LACBS_01513) - 1530031..1533591 (-) 3561 WP_056936408.1 PD-(D/E)XK nuclease family protein -
  LEUCM_RS07640 (LACBS_01514) mvk 1533868..1534815 (+) 948 WP_025015793.1 mevalonate kinase -

Sequence

Protein


Download         Length: 252 a.a.        Molecular weight: 28726.53 Da        Isoelectric Point: 8.0083

>NTDB_id=57717 LEUCM_RS10015 WP_035146927.1 1529280..1530038(-) (addA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MSNPQFTPSQQAAVDHDGHDILVSASAGSGKTSVLVARVIQKILNGTDVDTLLVVTFTEAAATEMRQRIQVALRDASEKA
TEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYNHEYVKENEQ
DTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNNLPKSYAVDEAGLTASDYFQKKYYRFYSKPSPNVWKPY
KKLNSSVKRAKI

Nucleotide


Download         Length: 759 bp        

>NTDB_id=57717 LEUCM_RS10015 WP_035146927.1 1529280..1530038(-) (addA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
TTGAGTAACCCCCAATTTACACCTTCACAGCAAGCCGCTGTCGATCATGATGGCCACGATATTTTAGTATCCGCATCAGC
TGGTTCAGGGAAGACATCCGTTCTTGTTGCGCGGGTCATCCAAAAAATCCTAAACGGGACCGATGTCGATACCTTACTTG
TTGTGACGTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGTAGCACTCCGCGACGCCAGTGAGAAAGCA
ACAGAACCAGCCGTTAAGCAACGCTTACGGCAACAATTAAGTCTCGTGCCTACCGCACAAATTAGTACACTGCATGCCTT
TTGTTTAAAAGTCATTAAACAATTTTATTATGTCATTGATCGTGATCCCGTATTCCGCTTATTGAGTGATACCGCAGAAC
GACTCCTATTAGCGGATCAAGTATGGCAACGGGTGAGAGAGGCCTTCTATAATCACGAATATGTCAAAGAAAATGAGCAA
GATACATTATTCTACGAATTAGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTGACCGACATTGTTTTTGAGTT
GCTCGATTTTGCAAACGCCAATCCAAACCCGGTTAAATGGTTAAATAACCTTCCCAAAAGTTATGCCGTTGACGAAGCTG
GTTTGACGGCCAGTGATTATTTCCAAAAAAAATATTACCGATTTTACAGCAAACCATCACCGAATGTTTGGAAGCCTTAC
AAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAA

Domains

Predicted by InterProScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(7-229)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

42.523

84.921

0.361

Multiple sequence alignment