Detailed information NTG0057698

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LEUCM_RS07630
• Genome accession: NZ_AP017929
• Coordinates: 1526305..1529331 (-)
• Length: 1008 a.a.
• NCBI ID: WP_258421054.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1521305..1534331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LEUCM_RS07615 (LACBS_01508)	asnS	1521442..1522740 (-)	1299	WP_011374616.1	asparagine--tRNA ligase	-
LEUCM_RS07620 (LACBS_01509)	-	1522851..1523327 (-)	477	WP_011374615.1	DUF5590 domain-containing protein	-
LEUCM_RS07625 (LACBS_01510)	-	1523405..1526239 (-)	2835	WP_016265073.1	helicase C-terminal domain-containing protein	-
LEUCM_RS07630 (LACBS_01511)	addA	1526305..1529331 (-)	3027	WP_258421054.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LEUCM_RS10015 (LACBS_01512)	addA	1529280..1530038 (-)	759	WP_035146927.1	UvrD-helicase domain-containing protein	Machinery gene
LEUCM_RS07635 (LACBS_01513)	-	1530031..1533591 (-)	3561	WP_056936408.1	PD-(D/E)XK nuclease family protein	-

Sequence

Protein

• Length: 1008 a.a.
• Molecular weight: 114660.96 Da
• Isoelectric Point: 4.7595

>NTDB_id=57698 LEUCM_RS07630 WP_258421054.1 1526305..1529331(-) (addA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
MEALQEAEQLSQTSENLMIYAPQIATTQAALTQINADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLD
DVKKKIQTLLDTYFVFDEATTANIQRQAGQLVQKLVSVTLTFRAAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFY
QQKFSEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENF
RSRRNIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKR
IQAMMTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQ
DIPLVSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVD
LIWTIYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTI
HGSKGLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTR
AEQRLIIVGHCDNQAQLMKQWQKGQNSTQQILDDGVRNDAASLLDWIGLSLIRHPQAKDWTTDYEPLLALSGDQTEFKLI
MTNETLLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQ
LDLAAEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQALVDRLVLQNSLSANVAAKIN
LNHLLTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSD
QEAAIERVKTRYAGQINLYSAALNQIAQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3027 bp

>NTDB_id=57698 LEUCM_RS07630 WP_258421054.1 1526305..1529331(-) (addA) [Latilactobacillus sakei subsp. sakei DSM 20017 = JCM 1157 strain LT-13]
TTGGAAGCCTTACAAGAAGCTGAACAGCTCAGTCAAACGAGCGAAAATCTAATGATCTATGCCCCTCAAATTGCAACTAC
GCAAGCAGCCTTAACACAAATTAACGCAGATGCAAAAACGTTGAATTGGCAAGCATGGCGCCAACAACTAACAGATGCAC
AGCTGGGGCCTGCTAAACGGACTAAGAAATTAGAACCCGACGAACAAATTAATAAGGACCGGTTGAAAGCCGATTTAGAT
GACGTCAAAAAGAAAATTCAGACGTTACTGGACACCTATTTTGTGTTTGATGAAGCAACCACCGCTAATATTCAACGTCA
AGCTGGCCAATTGGTTCAAAAATTGGTGAGCGTCACGTTAACTTTTAGAGCAGCCTTTCAAGCCGAAAAAGAACGACGTC
ATTTATTGGACTTTAGTGATTTAGAACAACTTTGTCTGACAATTTTATCGGCAGAGGATTCGCCGGCAAGGGCCTTTTAC
CAACAAAAATTTAGCGAAGTGCTAGTTGATGAATATCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCAC
TTCTGATCATCCACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCT
TATTTAAGAATAAGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTC
CGTTCGCGCCGCAATATCGATGACTTTACCAATCTGATTTTTAAACAATTGATGGACGAAAATCTCGGCGAGTTAGACTA
CGACGAAAATGCGGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTT
ATGAAACTAAGCCGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGG
ATTCAAGCCATGATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACAT
CGTTTTACTAGCCCCAACCCGTGGTAATAATTTGTTTATCCTGGATTATTTCAAACGCTTCGGTTTACCGGTTGTGATTA
AGGATGCGCAAAACTATTTCCAAACGACCGAAGTCCAAATTATGTTAGCTCTACTTCAGGTGATTGATAATCCTAACCAA
GATATTCCATTGGTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGA
TAAGACGGATGATTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAACGAGCAGAAAGTCGGGCATTTAGGCCAAC
AGGTCATGCAGAAGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATTGCTCGCCAACAACCGATTGTGGAC
TTAATTTGGACGATCTACCAAGAAACTGGCTTTTTAGACTATGTCGGTGGGATGCCAGCTGGACGTCAACGTCAAGCTAA
TTTACACGCGCTTTATGAACGTGCCACGGCGTATGAAGAAAATGGCTTTAAAGGACTCTTCCAATTTATTCAATTTATTG
AACGTCTCCAGAAACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAGGACGCCATCTCTGTTATGACCATT
CACGGGAGTAAGGGGCTTGAATTTCCAGTCGTCTTTTTAATCGATACGAGTCGTCGCTTTAACCAACAGGATTTACAACG
CTCATATGTTTTGGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTGCCAACACTGC
CAGAGTTAGCGATTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGT
GCGGAACAACGGTTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAG
TACCCAACAAATATTAGATGATGGGGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCC
ATCCACAAGCTAAAGATTGGACCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAATTTAAGTTGATT
ATGACTAATGAAACCTTACTTGGTGAAATACCAGGTAGTCTGCATAACGATCAGGACTGGGGACAACAACAGCAAAAGGC
ACTTCAAACAATTGATTTAAACAGTGGGATAGCAACTGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTG
CCACCCAAACGACGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGGTTGGGACAA
TTAGACCTAGCGGCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAAC
CGTTCAACAACCAACGGCAGCAGAAGTAGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAA
CGCTAGCGAGTTTGCAAGCCCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAAT
CTTAATCACCTATTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGT
GCCATTTTCGTTGTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGATCACGTCCTAATCCATG
GGATTATGGATGGTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGAT
CAAGAAGCGGCGATTGAACGGGTTAAAACCCGCTATGCTGGCCAGATTAACCTCTATAGTGCTGCTTTAAACCAGATTGC
CCAAAAGCCAGTGACCCATAAATATCTCTATTTATTGTCAATTGGCGAGTTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (26-222)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (828-991)

• UvrD-like helicase C-terminal domain (UvrD_C) (234-648)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	38.778	94.147	0.365