Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   KPG64_RS23145 Genome accession   NZ_CP076539
Coordinates   4517117..4519456 (-) Length   779 a.a.
NCBI ID   WP_016077246.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain GR007     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4512117..4524456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPG64_RS23120 (KPG64_23120) - 4512999..4513175 (-) 177 WP_001242605.1 hypothetical protein -
  KPG64_RS23125 (KPG64_23125) - 4513258..4513590 (-) 333 WP_000938751.1 YrdB family protein -
  KPG64_RS23130 (KPG64_23130) - 4513706..4514146 (-) 441 WP_001266969.1 D-tyrosyl-tRNA(Tyr) deacylase -
  KPG64_RS23135 (KPG64_23135) relA 4514163..4516346 (-) 2184 WP_001262795.1 GTP diphosphokinase -
  KPG64_RS23140 (KPG64_23140) - 4516557..4517069 (-) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  KPG64_RS23145 (KPG64_23145) recJ 4517117..4519456 (-) 2340 WP_016077246.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  KPG64_RS23150 (KPG64_23150) - 4519558..4520451 (-) 894 WP_000409739.1 cation diffusion facilitator family transporter -
  KPG64_RS23155 (KPG64_23155) secDF 4520608..4522872 (-) 2265 WP_016077245.1 protein translocase subunit SecDF -
  KPG64_RS23160 (KPG64_23160) - 4523142..4523426 (-) 285 WP_000884549.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88150.64 Da        Isoelectric Point: 4.8429

>NTDB_id=576350 KPG64_RS23145 WP_016077246.1 4517117..4519456(-) (recJ) [Bacillus thuringiensis strain GR007]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTEEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLEDYKEKLMHASEVTHLDDQYIVLLDLPNGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=576350 KPG64_RS23145 WP_016077246.1 4517117..4519456(-) (recJ) [Bacillus thuringiensis strain GR007]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCGAATGTAGCAAAGGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGATGGCGGTGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATCGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAAAAATATCGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATTGCACCGCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAACGC
CGGCTGTACACCTTTTATTATCAGATGATCCTGAGGAAGCGAAAGAGTTGGCCGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACCGAAGAGGCTATCGCTGAAGTTGAAAATAACTTTCCGCCAGAAGAAAACAAAGT
ATTAGTACTTGCAAAAGAAGGTTGGAATCCAGGTGTAATTGGGATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACTATTGTATTATGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCGCGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTGCCACACTTCGGAGGGCATCCGATGGCAGCTGGGATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGTCGCTTAAATGAACAGGCAGATGCAATTTTAACAGAAGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGTAAAGTGGAGGATGTAACGCTAGCGGCAATTGAAGATATGCAGAAGTTAGCACCATTTGGTGTT
GGAAATCCGAAACCACGTATTGCAGTGAAAGATGCAGAGTTAGAAAGTATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTCGGTTTCGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCATCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAACTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGGACTATAAAGAAAAGCTTATGC
ATGCGTCAGAAGTAACGCATTTAGATGATCAATACATCGTGTTACTCGATTTGCCAAACGGAACAGATGAATTACGTGAG
TTATTTAAAGTAGGATTCCCAGCGCGAATTTATACGCTGTTTTATCAAGAAAATAACCATCTATTTAGTACAGTTCCTAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.455

99.358

0.521