Detailed information    

experimental Experimentally validated

Overview

Name   comFC   Type   Machinery gene
Locus tag   C694_RS07590 Genome accession   NC_018939
Coordinates   1546794..1547369 (-) Length   191 a.a.
NCBI ID   WP_001203580.1    Uniprot ID   A0AAC8SBK6
Organism   Helicobacter pylori 26695     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

Helicobacter pylori ComFC is involved in DNA transport through the cell membrane, and is required for the handling of the single-stranded DNA once it is delivered into the cytoplasm. The crystal structure of ComFC includes a zinc-finger motif and a putative phosphoribosyl transferase domain, both necessary for the protein's in vivo activity. ComFC is a membrane-associated protein with affinity for single-stranded DNA.


Genomic Context

Location: 1541794..1552369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS07580 (C694_07615) - 1543465..1544676 (-) 1212 WP_000502610.1 restriction endonuclease subunit S -
  C694_RS07585 (C694_07620) - 1544724..1546763 (-) 2040 WP_001045361.1 VRR-NUC domain-containing protein -
  C694_RS07590 (C694_07625) comFC 1546794..1547369 (-) 576 WP_001203580.1 ComF family protein Machinery gene
  C694_RS07595 (C694_07630) tmk 1547357..1547932 (-) 576 WP_000289698.1 dTMP kinase -
  C694_RS07600 (C694_07635) coaD 1547934..1548407 (-) 474 WP_001169234.1 pantetheine-phosphate adenylyltransferase -
  C694_RS07605 (C694_07640) - 1548407..1548970 (-) 564 WP_000780126.1 UbiX family flavin prenyltransferase -
  C694_RS07610 (C694_07650) flgA 1548980..1549636 (-) 657 WP_000697639.1 flagellar basal body P-ring formation chaperone FlgA -
  C694_RS07615 (C694_07655) uvrD 1549633..1551681 (-) 2049 WP_000968580.1 DNA helicase UvrD -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 21331.91 Da        Isoelectric Point: 8.8889

>NTDB_id=1221 C694_RS07590 WP_001203580.1 1546794..1547369(-) (comFC) [Helicobacter pylori 26695]
MRCLTCLKLSFKPLCPNCLNDLPLSLKVRVLEGVSVYSFYAYSEIEELIKSKYALIGSRILPLLSQKAGAEFVKILQEQG
LNIPLYGIAIDDKIKSFYSHSAALLKGFCQGNLKPTYGRLRANNAVSYAGKSLEFRANNPRNFTFKGDESLDYFLLDDII
TTGTTLKEALKYLKTLNIKVHFAIALCSADE

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1221 C694_RS07590 WP_001203580.1 1546794..1547369(-) (comFC) [Helicobacter pylori 26695]
ATGCGCTGTTTAACCTGTTTGAAGCTTTCTTTTAAGCCTCTTTGCCCAAATTGCTTGAACGATTTGCCCTTAAGCTTAAA
GGTAAGGGTTTTAGAGGGCGTGAGCGTGTATAGTTTTTACGCTTATAGCGAAATAGAAGAGCTCATTAAAAGCAAATACG
CGCTGATTGGCTCTCGCATTTTGCCCTTGCTTTCTCAAAAAGCCGGTGCAGAGTTTGTGAAAATCCTGCAAGAACAAGGC
TTGAATATCCCCCTTTATGGCATCGCCATTGATGATAAAATCAAATCCTTTTACTCGCATTCCGCCGCGCTTTTAAAAGG
CTTTTGTCAAGGGAATTTAAAGCCCACTTACGGGCGTTTAAGGGCTAATAATGCTGTTTCGTATGCCGGGAAAAGCTTAG
AATTTCGCGCCAACAACCCACGGAATTTCACCTTCAAAGGCGATGAAAGTTTAGATTATTTTTTACTAGATGATATTATC
ACCACCGGCACCACCCTAAAAGAAGCCCTAAAATACCTTAAAACCCTAAACATAAAAGTACACTTTGCGATCGCGCTTTG
CAGCGCGGATGAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ctsW Campylobacter jejuni subsp. jejuni 81-176

36.979

100

0.372

Multiple sequence alignment    



References

[1] Prashant P Damke et al. (2022) ComFC mediates transport and handling of single-stranded DNA during natural transformation. Nature Communications 13(1):1961. [PMID: 35414142]
[2] K C Chang et al. (2001) Identification of genes associated with natural competence in Helicobacter pylori by transposon shuttle random mutagenesis. Biochemical And Biophysical Research Communications 288(4):961-8. [PMID: 11689003]