Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KOM03_RS06930 Genome accession   NZ_CP076450
Coordinates   1349176..1349613 (-) Length   145 a.a.
NCBI ID   WP_060563062.1    Uniprot ID   -
Organism   Bacillus velezensis strain GMEKP1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1344176..1354613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOM03_RS06905 (KOM03_06905) - 1345195..1346331 (+) 1137 WP_052827030.1 zinc-dependent alcohol dehydrogenase -
  KOM03_RS06910 (KOM03_06910) - 1346346..1346780 (+) 435 WP_007409358.1 RDD family protein -
  KOM03_RS06915 (KOM03_06915) - 1346852..1347175 (+) 324 WP_060563059.1 YckD family protein -
  KOM03_RS06920 (KOM03_06920) - 1347280..1348716 (+) 1437 WP_060563060.1 family 1 glycosylhydrolase -
  KOM03_RS06925 (KOM03_06925) nin/comJ 1348757..1349155 (-) 399 WP_060563061.1 competence protein ComJ Regulator
  KOM03_RS06930 (KOM03_06930) nucA/comI 1349176..1349613 (-) 438 WP_060563062.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KOM03_RS06935 (KOM03_06935) hxlB 1349972..1350529 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  KOM03_RS06940 (KOM03_06940) hxlA 1350526..1351161 (-) 636 WP_060563063.1 3-hexulose-6-phosphate synthase -
  KOM03_RS06945 (KOM03_06945) - 1351393..1351770 (+) 378 WP_207169049.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16086.38 Da        Isoelectric Point: 8.4850

>NTDB_id=575459 KOM03_RS06930 WP_060563062.1 1349176..1349613(-) (nucA/comI) [Bacillus velezensis strain GMEKP1]
MKTLKALLLILIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=575459 KOM03_RS06930 WP_060563062.1 1349176..1349613(-) (nucA/comI) [Bacillus velezensis strain GMEKP1]
TTGAAAACATTAAAAGCACTGCTGCTTATTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641