Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   KO461_RS00330 Genome accession   NZ_CP076449
Coordinates   64274..64825 (+) Length   183 a.a.
NCBI ID   WP_205764719.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain CU1000N     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 59274..69825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO461_RS00315 (KO461_00310) - 60223..61587 (+) 1365 WP_005564159.1 L-serine ammonia-lyase -
  KO461_RS00320 (KO461_00315) ansB 61769..62818 (-) 1050 WP_005561854.1 L-asparaginase 2 -
  KO461_RS00325 (KO461_00320) - 63092..64252 (+) 1161 WP_094978646.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  KO461_RS00330 (KO461_00325) pilF/pilF2 64274..64825 (+) 552 WP_205764719.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KO461_RS00335 (KO461_00330) - 64969..66024 (+) 1056 WP_205764718.1 helix-turn-helix domain-containing protein -
  KO461_RS00340 (KO461_00335) ispG 66036..67139 (+) 1104 WP_005564165.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KO461_RS00345 (KO461_00340) hisS 67161..68438 (+) 1278 WP_005561860.1 histidine--tRNA ligase -
  KO461_RS00350 (KO461_00345) - 68449..69063 (+) 615 WP_005561861.1 YfgM family protein -
  KO461_RS00355 (KO461_00350) - 69116..69568 (+) 453 WP_005539334.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20652.37 Da        Isoelectric Point: 7.6502

>NTDB_id=575412 KO461_RS00330 WP_205764719.1 64274..64825(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain CU1000N]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKACSYAPDYYLVSEAFAYF
YQQQGDIEQARAAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPERAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=575412 KO461_RS00330 WP_205764719.1 64274..64825(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain CU1000N]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAACAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTGTTCTTACGCTCCAGATTATTATTTAGTGTCAGAGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTGCCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTCCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTACGAAAACTTGGCGCTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCCCCGGAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

56.97

90.164

0.514
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437