Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   KM843_RS16535 Genome accession   NZ_CP076291
Coordinates   3373922..3375307 (-) Length   461 a.a.
NCBI ID   WP_012118573.1    Uniprot ID   A7Z9B6
Organism   Bacillus velezensis strain US1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3368922..3380307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM843_RS16500 (KM843_16500) flgL 3369097..3370014 (-) 918 WP_012118569.1 flagellar hook-associated protein FlgL -
  KM843_RS16505 (KM843_16505) flgK 3370026..3371543 (-) 1518 WP_012118570.1 flagellar hook-associated protein FlgK -
  KM843_RS16510 (KM843_16510) - 3371559..3372041 (-) 483 WP_003151394.1 flagellar protein FlgN -
  KM843_RS16515 (KM843_16515) flgM 3372056..3372322 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  KM843_RS16520 (KM843_16520) - 3372392..3372811 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  KM843_RS16525 (KM843_16525) comFC 3372885..3373574 (-) 690 WP_197197403.1 double zinc ribbon domain-containing protein Machinery gene
  KM843_RS16530 (KM843_16530) - 3373580..3373864 (-) 285 WP_007407481.1 competence protein ComFB -
  KM843_RS16535 (KM843_16535) comFA 3373922..3375307 (-) 1386 WP_012118573.1 DEAD/DEAH box helicase Machinery gene
  KM843_RS16540 (KM843_16540) - 3375415..3376263 (-) 849 WP_003151387.1 DegV family protein -
  KM843_RS16545 (KM843_16545) degU 3376361..3377050 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  KM843_RS16550 (KM843_16550) degS 3377127..3378290 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  KM843_RS16555 (KM843_16555) - 3378513..3379160 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52493.84 Da        Isoelectric Point: 10.2968

>NTDB_id=574568 KM843_RS16535 WP_012118573.1 3373922..3375307(-) (comFA) [Bacillus velezensis strain US1]
MSEGASEFSEVRDFFFGRHLLRSEVPFSDHHIKRVTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVVMGRADELTSLYSWNQAHENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSHLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRNGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITSGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=574568 KM843_RS16535 WP_012118573.1 3373922..3375307(-) (comFA) [Bacillus velezensis strain US1]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTTCGGCCGGCATTTATTGAGATCTGAGGTACCGTT
TTCAGATCATCACATCAAGCGTGTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCGTGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCATGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGTTAATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACATCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAAACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTCACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATCACAAGCGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z9B6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62