Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   KM776_RS18225 Genome accession   NZ_CP076290
Coordinates   3652948..3654333 (-) Length   461 a.a.
NCBI ID   WP_063174504.1    Uniprot ID   -
Organism   Bacillus velezensis strain Q12     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3647948..3659333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM776_RS18190 (KM776_18190) flgL 3648123..3649040 (-) 918 WP_069013671.1 flagellar hook-associated protein FlgL -
  KM776_RS18195 (KM776_18195) flgK 3649052..3650569 (-) 1518 WP_003151395.1 flagellar hook-associated protein FlgK -
  KM776_RS18200 (KM776_18200) - 3650585..3651067 (-) 483 WP_003151394.1 flagellar protein FlgN -
  KM776_RS18205 (KM776_18205) flgM 3651082..3651348 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  KM776_RS18210 (KM776_18210) - 3651418..3651837 (-) 420 WP_003151392.1 TIGR03826 family flagellar region protein -
  KM776_RS18215 (KM776_18215) comFC 3651911..3652600 (-) 690 WP_196616643.1 phosphoribosyltransferase family protein Machinery gene
  KM776_RS18220 (KM776_18220) - 3652606..3652890 (-) 285 WP_003151389.1 competence protein ComFB -
  KM776_RS18225 (KM776_18225) comFA 3652948..3654333 (-) 1386 WP_063174504.1 DEAD/DEAH box helicase Machinery gene
  KM776_RS18230 (KM776_18230) - 3654441..3655289 (-) 849 WP_014305929.1 DegV family protein -
  KM776_RS18235 (KM776_18235) degU 3655387..3656076 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  KM776_RS18240 (KM776_18240) degS 3656153..3657316 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  KM776_RS18245 (KM776_18245) - 3657539..3658186 (+) 648 WP_003151384.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52457.99 Da        Isoelectric Point: 10.2294

>NTDB_id=574494 KM776_RS18225 WP_063174504.1 3652948..3654333(-) (comFA) [Bacillus velezensis strain Q12]
MAEGASEFSEVRDFFYGRHLLRSEIPFSDHCIKRFIEKEHITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENRWKPVKLSWEGTLTDGQKQAAAALTDAIKGRQELLVWAVCGSGKTEMLFPGIEY
ALNQGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVLFHFGMTNGMKKAKKHIELMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=574494 KM776_RS18225 WP_063174504.1 3652948..3654333(-) (comFA) [Bacillus velezensis strain Q12]
ATGGCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCATTGCATCAAGCGTTTTATAGAAAAAGAGCATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAGAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
ATGGAAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGGGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTAT
GCCCTGAATCAAGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCTGA
CACATTAAAAAAGAATGCTGAAGCAGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGATCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCCTGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACTTATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(328-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.043

99.783

0.629