Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PCNPT3_RS01615 Genome accession   NC_020802
Coordinates   356710..357582 (+) Length   290 a.a.
NCBI ID   WP_015464129.1    Uniprot ID   M4U1H9
Organism   Psychromonas sp. CNPT3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351710..362582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCNPT3_RS01595 (PCNPT3_01510) cpdA 352558..353385 (+) 828 WP_015464125.1 3',5'-cyclic-AMP phosphodiesterase -
  PCNPT3_RS01600 (PCNPT3_01515) - 353385..353957 (+) 573 WP_015464126.1 YqiA/YcfP family alpha/beta fold hydrolase -
  PCNPT3_RS01605 (PCNPT3_01520) parE 354205..356094 (+) 1890 WP_015464127.1 DNA topoisomerase IV subunit B -
  PCNPT3_RS01610 (PCNPT3_01525) ndk 356267..356695 (+) 429 WP_015464128.1 nucleoside-diphosphate kinase -
  PCNPT3_RS01615 (PCNPT3_01530) pilD 356710..357582 (+) 873 WP_015464129.1 A24 family peptidase Machinery gene
  PCNPT3_RS01620 (PCNPT3_01535) tadA 357582..358085 (+) 504 WP_041771348.1 tRNA adenosine(34) deaminase TadA -
  PCNPT3_RS01625 (PCNPT3_01540) mltF 358129..360102 (-) 1974 WP_015464131.1 membrane-bound lytic murein transglycosylase MltF -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32510.28 Da        Isoelectric Point: 6.9694

>NTDB_id=57363 PCNPT3_RS01615 WP_015464129.1 356710..357582(+) (pilD) [Psychromonas sp. CNPT3]
MSDFLVLMDQIPALLYIMVTCLGLLIGSFLNVVIYRLPVMMERAWKAECAEYFPETKLIADEDTFNLILPRSRCPKCLHI
IGSLENIPIISWLLQRGKCKHCACKISARYPCVELLTAIMSLAVAYVIPFGWPLLFALIFTWVLICLSYIDFDVMLLPDQ
LTLPLLWVGLLVNLSYSFVSINDAVVGAILGYLSLWSVYWLFKLATGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
SGAIIGIVLLLLSKNKESKPLPFGPYLAIAGWIYLICGPLLTQYYYALIL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=57363 PCNPT3_RS01615 WP_015464129.1 356710..357582(+) (pilD) [Psychromonas sp. CNPT3]
ATGAGTGATTTTTTAGTTTTGATGGATCAAATACCCGCGCTGTTATACATCATGGTAACTTGTTTGGGATTACTCATAGG
ATCGTTTTTAAATGTAGTGATATACCGTTTACCCGTGATGATGGAGCGTGCATGGAAAGCGGAATGTGCTGAATATTTCC
CTGAAACAAAATTAATTGCAGACGAAGATACTTTTAATCTTATTTTGCCTCGTTCACGCTGTCCTAAATGTTTACATATT
ATTGGCTCCCTTGAAAACATTCCTATTATTAGCTGGTTATTACAACGCGGAAAATGTAAACACTGCGCTTGTAAAATAAG
TGCCCGTTATCCCTGTGTTGAGCTATTAACGGCTATTATGTCCTTGGCCGTTGCTTATGTTATTCCGTTCGGTTGGCCTC
TCCTCTTTGCTTTGATTTTTACTTGGGTATTGATTTGTCTGTCTTATATTGATTTTGATGTCATGTTATTACCTGATCAA
TTAACCTTGCCACTACTTTGGGTGGGTTTACTCGTCAATTTATCCTATAGTTTTGTGTCGATTAATGATGCTGTGGTGGG
GGCTATTTTGGGTTATTTAAGTCTATGGTCAGTATATTGGCTATTTAAGCTTGCCACGGGTAAAGAAGGCATGGGTTATG
GCGACTTTAAATTGTTAGCTGCATTAGGCGCTTGGTTTGGCTGGCAGGCTCTGCCTTTAATTATTTTGCTGTCTTCTTTT
TCAGGGGCAATTATCGGTATAGTCTTATTACTCTTATCTAAAAACAAAGAAAGTAAGCCGTTACCCTTCGGCCCTTATTT
AGCCATCGCGGGTTGGATTTATCTAATATGTGGCCCTTTATTAACGCAATATTACTATGCGTTGATTTTATAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-126)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M4U1H9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

58.123

95.517

0.555
  pilD Vibrio campbellii strain DS40M4

56.429

96.552

0.545
  pilD Acinetobacter baumannii D1279779

51.93

98.276

0.51
  pilD Acinetobacter nosocomialis M2

51.93

98.276

0.51
  pilD Neisseria gonorrhoeae MS11

50.584

88.621

0.448

Multiple sequence alignment